In Vitro Apoptotic Cell Death Assessment
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Data
2021-01-01
Autores
Pereira, Lilian Cristina [UNESP]
de Souza, Alecsandra Oliveira
Miranda, Raul Ghiraldelli
Dorta, Daniel Junqueira [UNESP]
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Resumo
Chemical compounds induce cytotoxicity by various mechanisms, including interference in membrane integrity, metabolism, cellular component degradation or release, and cell division. Between the classic death pathways, namely, autophagy, apoptosis, and necrosis, apoptosis have been in the focus for the last several years as an important pathway for the toxicity of different types of xenobiotics. Because of that, having the tools to evaluate it is key for understanding and explaining the toxicodynamics of different classes of substances. Here, we describe a wide array of classic assays that can be easily implemented to evaluate apoptosis induction.
Descrição
Palavras-chave
Annexin-V, Apoptosis, Cell death, Nuclear fragmentation, Phosphatidilserine exposure
Como citar
Methods in Molecular Biology, v. 2240, p. 243-261.