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dc.contributor.authorRichardi, Jessica Freitas [UNESP]
dc.contributor.authorKogawa, Ana Carolina [UNESP]
dc.contributor.authorBelavenuto, Eliane Gandolpho Tótoli [UNESP]
dc.contributor.authorChorilli, Marlus [UNESP]
dc.contributor.authorSalgado, Hérida Regina Nunes [UNESP]
dc.date.accessioned2021-06-25T11:01:05Z
dc.date.available2021-06-25T11:01:05Z
dc.date.issued2021-05-21
dc.identifierhttp://dx.doi.org/10.1093/jaoacint/qsaa112
dc.identifier.citationJournal of AOAC International, v. 104, n. 2, p. 466-471, 2021.
dc.identifier.issn1944-7922
dc.identifier.urihttp://hdl.handle.net/11449/207791
dc.description.abstractBACKGROUND: Physicochemical and microbiological methods are found in the literature for the analysis of daptomycin, an antimicrobial. OBJECTIVE: This paper brings a miniaturized turbidimetric microbiological method for analysis of daptomycin in lyophilized powder. METHODS: The method was performed using 96-well microplates, 4-h incubation, 2, 4 and 8 μg/mL, 7% Staphylococcus aureus ATCC 6538 IAL 2082, and BHI broth. RESULTS: Linearity was proven by obtaining analytical curves with a correlation coefficient greater than 0.99 and statistical evaluation by ANOVA. The method was also selective, since the standard and sample analytical curves were parallel, proving that the excipient does not interfere with daptomycin analysis. Intraday, interday and inter-analyst precision presented RSDs of 2, 2.27, and 1.08%, respectively. Accuracy was assessed by the recovery test, where known quantities of standard solution are added to the sample and an average recovery value of 100.73% (RSD = 0.71%) was obtained. The present method was robust when minor changes were made in the parameters of used antimicrobial volume, inoculum volume and incubation time. CONCLUSIONS: This work is an innovative and ecological proposal and has advantages such as (i) less waste generation, (ii) miniaturized quantities of sample, culture media and inoculum, (iii) no need to use formaldehyde as in the traditional turbidimetric method, (iv) lower volume of glassware used and (v) shorter incubation time compared to other methods as agar diffusion requiring approximately 24 h. HIGHLIGHTS: This work is focuses on a current, innovative and sustainable theme for pharmaceutical analysis around the world.en
dc.format.extent466-471
dc.language.isoeng
dc.relation.ispartofJournal of AOAC International
dc.sourceScopus
dc.titleAn Ecological and Miniaturized Biological Method for the Analysis of Daptomycin Potencyen
dc.typeArtigo
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Goiás (UFG)
dc.description.affiliationSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines
dc.description.affiliationUniversidade Federal de Goiás - UFG Faculdade de Farmácia Laboratório de Controle de Qualidade
dc.description.affiliationUnespSão Paulo State University (UNESP) School of Pharmaceutical Sciences Department of Drugs and Medicines
dc.identifier.doi10.1093/jaoacint/qsaa112
dc.identifier.scopus2-s2.0-85106712782
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