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dc.contributor.authorEly, Valessa Lunkes
dc.contributor.authorCosta, Mateus Matiuzzi Da
dc.contributor.authorOliveira, Helinando Pequeno De
dc.contributor.authorSilva Junior, Fernando Antonio Gomes Da
dc.contributor.authorPereira, Daniela Isabel Brayer
dc.contributor.authorSoares, Mauro Pereira
dc.contributor.authorVargas, Agueda Castagna De
dc.contributor.authorSangioni, Luis Antonio
dc.contributor.authorCargnelutti, Juliana Felipetto
dc.contributor.authorRibeiro, Marcio Garcia [UNESP]
dc.contributor.authorBotton, Sonia De Avila
dc.identifier.citationMedical Mycology, v. 58, n. 8, p. 1114-1119, 2020.
dc.description.abstractAlgae of the genus Prototheca are microorganisms involved in the occurrence of diseases in humans and animals. In bovine species, Prototheca spp. cause environmental mastitis, productive losses in dairy herds, mainly leading to the discard of infected cows. Currently, there are no effective anti-Prototheca spp. drugs to combat this infection. Thus, the search for an efficacious therapy for Prototheca spp. infections have become essential. Highly soluble polypyrrole (Ppy) is a molecule with known antimicrobial activity. This study aimed to characterize Prototheca spp. isolates from bovine mastitis as well as to evaluate the susceptibility profile and to verify the morphological alterations on Prototheca spp. isolates treated with Ppy. In this research, 36 Brazilian isolates of Prototheca spp. were characterized by restriction fragment length polymorphism polymerase chain reaction (RFLP-PCR) assay for the mitochondrial cytB gene. Additionally, Ppy algicidal activity against these isolates of Prototheca spp. was assessed by minimal microbicidal concentration method in microplates. Further, scanning electron microscopy (SEM) was performed in order to verify the morphological alterations on Prototheca spp. isolates in response to Ppy. The isolates were characterized as belonging to Prototheca zopfii genotype 2 (35/36) and Prototheca blaschkeae (1/36). Ppy had an algicidal effect on all isolates tested at concentrations ranging from 15.625 μg ml-1 to 62.5 μg ml-1. SEM showed changes on planktonic and sessile P. zopfii, including a decrease of the number of cells with the presence of an amorphous substance involving the cells. The algicidal activity of Ppy suggests the therapeutic potential of this molecule in the prevention and treatment of Prototheca spp. in bovine mastitis.en
dc.relation.ispartofMedical Mycology
dc.subjectalgicidal effect
dc.subjectPrototheca spp
dc.titleIn vitro algicidal effect of polypyrrole on Prototheca species isolates from bovine mastitisAlgicidal activity of polypyrrole on Prototheca spp.en
dc.contributor.institutionUniversidade Federal de Sergipe (UFS)
dc.contributor.institutionProjeto Irrigacao Senador Nilo Coelho S/n
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationPostgraduate Program in Veterinary Medicine (PPGMV) Department of Preventive Veterinary Medicine (DMVP) Federal University of Santa Maria (UFSM)., Av. Roraima, 1000, Predio 44, Sala 5007, Bairro Camobi
dc.description.affiliationFederal University of Sao Francisco Valley Projeto Irrigacao Senador Nilo Coelho S/n, Rodovia BR 407 - Km 12, Lote 543
dc.description.affiliationDepartament of Microbiology and Parasitology Instituto de Biologia Federal University of Pelotas Campus Universitario Capao Do Leao S/n, Predio 18, Sala 14.
dc.description.affiliationFaculty of Veterinary Medicine Federal University of Pelotas Campus Universitario Capao Do Leao S/n
dc.description.affiliationDepartment of Veterinary Hygiene and Public Health Sao Paulo State University - UNESP
dc.description.affiliationUnespDepartment of Veterinary Hygiene and Public Health Sao Paulo State University - UNESP
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