Complete Chromosome-scale genome sequence resource for sporisorium panici-leucophaei, the causal agent of sourgrass smut disease
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2021-04-01
Autores
Crestana, Gustavo Schiavone
Taniguti, Lucas Mitsuo
Dos Santos, Clesivan Pereira
Benevenuto, Juliana
Ceresini, Paulo Cezar [UNESP]
Carvalho, Giselle
Kitajima, João Paulo
Monteiro-Vitorello, Claudia Barros
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Here, we present the first complete chromosome-level genome assembly of the smut fungus strain Sporisorium panici-leucophaei SPL10A, the causal agent of the sourgrass (Digitaria insularis) smut disease. Combining Illumina paired-end and Nanopore long reads, we generated a final assembly composed of 23 chromosomes (22 nuclear and one mitochondrial) with 18,915,934 bp. Gene prediction accomplished using extrinsic evidence from the sugarcane smut fungus Sporisorium scitamineum originated a total of 6,402 protein-encoding genes. The secretome (388 proteins) and the effectorome repertoires (68 candidates) were also predicted, given their crucial roles in plant-pathogen interactions. The complete telomere-to-telomere chromosome sequences of this poorly studied fungus will provide a valuable resource for future comparative genomic studies among smuts to unravel their underlying pathogenicity mechanisms.
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Genome assembly, Nanopore sequencing, Plant-pathogen interaction, Smut fungi, Sporisorium scitamineum
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Molecular Plant-Microbe Interactions, v. 34, n. 4, p. 448-452, 2021.