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Identification and complete sequencing of novel human transcripts through the use of mouse orthologs and testis cDNA sequences
(Genetics and Molecular Research, 2004-12-01) [Trabalho apresentado em evento]
The correct identification of all human genes, and their derived transcripts, has not yet been achieved, and it remains one of the major aims of the worldwide genomics community. Computational programs suggest the existence ...
Identification of human chromosome 22 transcribed sequences with ORF expressed sequence tags
(Proceedings of the National Academy of Sciences of the United States of America, 2000-11-07) [Artigo]
Transcribed sequences in the human genome can be identified with confidence only by alignment with sequences derived from cDNAs synthesized from naturally occurring mRNAs. We constructed a set of 250,000 cDNAs that represent ...
A transcript finishing initiative for closing gaps in the human transcriptome
(Genome Research, 2004-07-01) [Artigo]
We report the results of a transcript finishing initiative, undertaken for the purpose of identifying and characterizing novel human transcripts, in which RT-PCR was used to bridge gaps between paired EST Clusters, mapped ...