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Artigo

Biochemical and Functional Characterization of a Metalloprotease from the Thermophilic Fungus Thermoascus aurantiacus

Merheb-Dini, Carolina [UNESP]; Cabral, Hamilton [UNESP]; Leite, Rodrigo S. R. [UNESP]; Zanphorlin, Leticia M. [UNESP]; Okamoto, Debora N. [UNESP]; Bonilla-Rodriguez, Gustavo Orlando [UNESP]; Juliano, Luiz; Arantes, Eliane C.; Gomes, Eleni [UNESP]; da Silva, Roberto [UNESP] (Journal of Agricultural and Food Chemistry, 2009-10-14) [Artigo]

Protease production was carried out in solid state fermentation. The enzyme was purified through precipitation with ethanol at 72% followed by chromatographies in columns of Sephadex G75 and Sephacryl S100. It was purified ...