Efeito do consumo de vinho tinto no desenvolvimento da periodontite apical induzida em ratos.
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Data
2021-08-03
Autores
Dal Fabbro, Renan [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
Objetivo: Avaliar o efeito do consumo de vinho tinto ou de seus polifenóis nos processos de inflamação / reabsorção associados à periodontite periapical (PP) em ratos. Metodologia: Trinta e dois ratos Wistar com 3 meses de idade tiveram a periodontite periapical induzida nos quatro primeiros molares, dispostos em quatro grupos: controle (C) - ratos com periodontite periapical; vinho (W) - ratos com PP recebendo 4,28 mL/kg de vinho tinto; resveratrol + quercetina (R+Q) - ratos com PP recebendo 4,28 mL/kg de solução contendo 1,00 mg/L de quercetina e 0,86 mg/L de resveratrol; e álcool (ALC) - ratos com PP recebendo a mesma dose alcoólica contida no vinho. Os tratamentos por gavagem foram administrados diariamente, do início ao 45o dia. No 15o dia a PP foi induzida e no 45o dia os animais foram eutanasiados. Foram realizadas análises histológicas, imunohistoquímica para RANKL, OPG, TRAP, IL-10, TNF-⍺ e IL-1β e por microtomografia computadorizada nas mandíbulas. O teste de Kruskal-Wallis com Dunn's foi realizado para dados não paramétricos e o teste ANOVA com Tukey's para dados paramétricos, p <0,05. Resultados: A mediana do escore do processo inflamatório foi menor no grupo R + Q (1) em comparação aos grupos C (2) e ALC (3), e não diferente do grupo W (1.5). Embora os grupos W e R+Q tenham apresentado menor pontuação para RANKL, TNF-⍺ e IL-1β, não foram encontradas diferenças em relação aos grupos C e ALC. A marcação imunológica para OPG foi maior no grupo R+Q em comparação com todos os grupos; o mesmo observado para IL-10, sendo diferente dos grupos C e ALC. O grupo R+Q apresentou a menor contagem de células TRAP, seguido pelo grupo W, ambos inferiores aos grupos C e ALC, que apresentaram os piores resultados. O menor valor de reabsorção óssea foi no grupo R+Q (0,50 mm3 ± 0,21 mm3), inferior ao grupo C (0,88 mm3 ± 0,10 mm3). O grupo W (0,60 mm3 ± 0,25 mm3) e o grupo R+Q apresentaram menor reabsorção óssea em comparação com o grupo ALC (0,97 mm3 ± 0,22 mm3). Conclusão: A administração de vinho tinto reduziu a inflamação oriunda da PP, a marcação TRAP e a reabsorção óssea periapical em comparação ao ALC; a administração de resveratrol-quercetina reduziu o processo inflamatório da PP, a reabsorção óssea periapical e alterou a expressão de OPG, IL-10 e TRAP em comparação aos grupos C e ALC.
Aim: To evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Thirty-two 3-month-old Wistar rats had the PP induced in the four first molars, arranged into four groups: control (C) - rats with PP; wine (W) - rats with PP receiving 4.28 mL/kg of red wine; resveratrol+quercetin (R+Q) - rats with PP receiving 4.28 mL/kg of solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol; and alcohol (ALC) - rats with PP receiving the alcoholic dose contained in the wine. The oral gavage treatments were daily administered, from day 0 to day 45th. At the 15th day the PP was induced, and at the 45th day the animals were euthanized; histological, immunohistochemical (RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β), and micro-computed tomography analysis were performed in the jaws. The Kruskal-Wallis with Dunn's test was performed for nonparametric data, and the ANOVA with Tukey's test for parametric data, p<0.05. Results: The median score of inflammatory process was statistically lower in the R+Q group (1) compared to the C (2) and ALC (3) groups, and not different from the W (1.5) group. Although the W and R+Q groups had a lower score for RANKL, TNF-⍺ and IL-1β, no differences were found compared to C and ALC. The immunolabelling for OPG was higher in the R+Q group compared to all groups; the same observed for IL-10, being different from groups C and ALC. The R+Q group had the lowest TRAP cell count, followed by the W group, both inferior to C and ALC groups, which presented the worst results. The lowest bone resorption value was in the R+Q group (0.50mm3±0.21mm3), statically lower than the C group (0.88mm3±0.10mm3). The W group (0.60 mm3±0.25 mm3) and R+Q group showed less bone resorption compared to the ALC group (0.97mm3±0.22mm3). Conclusion: Red wine administration led to lowers PP inflammation, TRAP marking, and periapical bone resorption compared to ALC; resveratrol-quercetin administration reduced the PP inflammatory processes, periapical bone resorption, and altered the OPG, IL-10, and TRAP expression compared to C and ALC groups.
Aim: To evaluate the effect of red wine consumption or its polyphenols on the inflammation/resorption processes associated with periapical periodontitis (PP) in rats. Methodology: Thirty-two 3-month-old Wistar rats had the PP induced in the four first molars, arranged into four groups: control (C) - rats with PP; wine (W) - rats with PP receiving 4.28 mL/kg of red wine; resveratrol+quercetin (R+Q) - rats with PP receiving 4.28 mL/kg of solution containing 1.00 mg/L of quercetin and 0.86 mg/L of resveratrol; and alcohol (ALC) - rats with PP receiving the alcoholic dose contained in the wine. The oral gavage treatments were daily administered, from day 0 to day 45th. At the 15th day the PP was induced, and at the 45th day the animals were euthanized; histological, immunohistochemical (RANKL, OPG, TRAP, IL-10, TNF-⍺ and IL-1β), and micro-computed tomography analysis were performed in the jaws. The Kruskal-Wallis with Dunn's test was performed for nonparametric data, and the ANOVA with Tukey's test for parametric data, p<0.05. Results: The median score of inflammatory process was statistically lower in the R+Q group (1) compared to the C (2) and ALC (3) groups, and not different from the W (1.5) group. Although the W and R+Q groups had a lower score for RANKL, TNF-⍺ and IL-1β, no differences were found compared to C and ALC. The immunolabelling for OPG was higher in the R+Q group compared to all groups; the same observed for IL-10, being different from groups C and ALC. The R+Q group had the lowest TRAP cell count, followed by the W group, both inferior to C and ALC groups, which presented the worst results. The lowest bone resorption value was in the R+Q group (0.50mm3±0.21mm3), statically lower than the C group (0.88mm3±0.10mm3). The W group (0.60 mm3±0.25 mm3) and R+Q group showed less bone resorption compared to the ALC group (0.97mm3±0.22mm3). Conclusion: Red wine administration led to lowers PP inflammation, TRAP marking, and periapical bone resorption compared to ALC; resveratrol-quercetin administration reduced the PP inflammatory processes, periapical bone resorption, and altered the OPG, IL-10, and TRAP expression compared to C and ALC groups.
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Palavras-chave
Vinho, Resveratrol, Quercetina, Polifenóis, Periodontite periapical, Periapical periodontitis