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dc.contributor.authorPinto, Maria Etelvina
dc.contributor.authorEgydio, Fernanda de Mattos [UNESP]
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorMendis-Handagama, S. M. L. Chamindrani
dc.contributor.authorGoes, Rejane Maira [UNESP]
dc.identifier.citationMicroscopy Research and Technique. Hoboken: Wiley-liss, v. 73, n. 2, p. 119-127, 2010.
dc.description.abstractInformation on postnatal Leydig cell (LC) differentiation in the Mongolian gerbil has been unavailable. Therefore, current investigation was designed to examine LC lineage differentiation in this rodent, from birth to adulthood. Gerbil testes at I day, 1-7 weeks (w), 2 and 3 months of age were conventionally processed by light and transmission electron microscopy. Immunocytochemistry for specific markers of steroidogenic enzymes, namely 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and 11 beta-hydroxysteroid steroid dehydrogenase 1 (11 beta-HSD1) and also for androgen receptor (AR) was performed. The establishment of adult Leydig cell populations (ALC) during testis maturation in the gerbil follows the pattern previously described in other mammalian species, with the four progressive stages of differentiation. The LC progenitors were identified at second w by 3 beta-HSD expression; the first newly formed ALC were recognized at fourth w whereas immature ALC appeared at fifth w. The latter were recognized by abundance of cytoplasmic lipid, in addition to expression of 11 beta-HSD1 and intense nuclear AR immunoreaction. Mature ALC in gerbil exhibited irregular eccentric nuclei and a cytoplasmic canaliculus in the perinuclear area. Only one third of mature ALC in adult gerbils showed a high expression of 11 beta-HSD1 and AR expression was highly variable among them. In conclusion, the process of differentiation of ALC population in gerbil follows the pattern previously established for other rodents. However, the resulting mature ALC are strikingly different due their singular asymmetric morphology and presence of a cytoplasmic canaliculus and as well as their functional heterogeneity. Microsc. Res. Tech. 73:119-127, 2010. (C) 2009 Wiley-Liss, Inc.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.relation.ispartofMicroscopy Research and Technique
dc.sourceWeb of Science
dc.subjectLeydig cellsen
dc.subjectMongolian gerbilen
dc.subjectmature adult Leydig cellsen
dc.titleDifferentiation of Leydig Cells in the Mongolian Gerbilen
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniv Tennessee
dc.description.affiliationUniv Estadual Campinas, Dept Cell Biol, Inst Biol, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Biol, IBILCE, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Tennessee, Dept Comparat Med, Coll Vet Med, Knoxville, TN 37996 USA
dc.description.affiliationUnespSão Paulo State Univ, Dept Biol, IBILCE, UNESP, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 06-07008
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
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