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dc.contributor.authorRibeiro, Daniele Lisboa [UNESP]
dc.contributor.authorPinto, Maria Etelvina [UNESP]
dc.contributor.authorRafacho, Alex [UNESP]
dc.contributor.authorBosqueiro, Jose Roberto
dc.contributor.authorMaeda, Samantha Yuri [UNESP]
dc.contributor.authorAnselmo-Franci, Janete Aparecida
dc.contributor.authorTaboga, Sebastiao Roberto [UNESP]
dc.contributor.authorGoes, Rejane Maira [UNESP]
dc.identifier.citationJournal of Andrology. Lawrence: Amer Soc Andrology, Inc, v. 33, n. 5, p. 854-865, 2012.
dc.description.abstractIn this study, we evaluated the effects of obesity and insulin resistance induced by a high-fat diet on prostate morphophysiology, focusing on cell proliferation, expression of androgen (AR) and estrogen receptors (ER) and proteins of the insulin signaling pathway. Adult male Wistar rats were fed a high-fat diet (20% fat) for 15 weeks, whereas control animals received a balanced diet (4% fat). Both groups were then divided and treated for 2 weeks with 1 mg/kg body weight/day of the aromatase inhibitor letrozole or vehicle only. The ventral prostate was analyzed with immunohistochemical, histopathological, stereological, and Western blotting methods. Obese rats showed insulin resistance, hyperinsulinemia, and reduced plasma testosterone levels. The incidence of prostatic intraepithelial neoplasia (PIN) was 2.7 times higher in obese rats and affected 0.4% of the gland compared with 0.1% PIN areas found in control rats. Obesity doubled cell proliferation in both prostate epithelium and stroma. AR content decreased in the prostate of obese rats and estrogen receptor beta (ER beta) increased in this group. Protein levels of insulin receptor substrate 1 and protein kinase B diminished in the obese group, whereas phosphatidylinositol 3-kinase (PI3K) increased significantly. Most structural changes observed in the prostate of obese rats normalized after letrozole treatment, except for increased stromal cell proliferation and ER beta expression, which might be associated with insulin resistance. This experimental model of obesity and insulin resistance induced by a high-fat diet increases cell proliferation in rat prostate. Such alterations are associated with decreased levels of AR and increased ER beta and PI3K proteins. This change can facilitate the establishment of proliferative lesions in rat prostate.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.publisherAmer Soc Andrology, Inc
dc.relation.ispartofJournal of Andrology
dc.sourceWeb of Science
dc.subjectfatty dieten
dc.subjectcell hyperplasiaen
dc.titleHigh-Fat Diet Obesity Associated With Insulin Resistance Increases Cell Proliferation, Estrogen Receptor, and PI3K Proteins in Rat Ventral Prostateen
dcterms.rightsHolderAmer Soc Andrology, Inc
dc.contributor.institutionUniversidade Federal de Uberlândia (UFU)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Federal de Santa Catarina (UFSC)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUniversidade Federal de Uberlândia (UFU), Inst Biomed Sci, Sect Histol, BR-38400 Uberlandia, MG, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Inst Biosci Letters & Exact Sci, Dept Biol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista UNESP, Sch Sci, Dept Phys Educ, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de Santa Catarina (UFSC), Ctr Biol Sci, Dept Physiol Sci, Florianopolis, SC, Brazil
dc.description.affiliationUniv São Paulo, Sch Dent, Dept Morphol Stomatol & Physiol, BR-09500900 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Inst Biosci Letters & Exact Sci, Dept Biol, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista UNESP, Sch Sci, Dept Phys Educ, São Paulo, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdFAPESP: 08/05341-2
dc.description.sponsorshipIdFAPESP: 08/00542-0
dc.description.sponsorshipIdFAPESP: 09/05078-2
dc.description.sponsorshipIdCNPq: 302693/2008-4
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
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