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dc.contributor.authorGiese, Ellen Cristine [UNESP]
dc.contributor.authorMonteiro, Alexandre C. [UNESP]
dc.contributor.authorBarbosa, Aneli de Melo
dc.contributor.authorDekker, Robert F. H.
dc.contributor.authordos Santos, Osvaldo
dc.contributor.authorCorradi da Silva, Maria de Lourdes
dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorda Silva, Roberto [UNESP]
dc.identifier.citationCurrent Research Topics In Applied Microbiology and Microbial Biotechnology. Singapore: World Scientific Publ Co Pte Ltd, p. 438-441, 2009.
dc.description.abstractBotryosphaeran, a new exopolysaccharide from the endophytic fungus Botryosphaeria rhodina MAMB-05, and algal laminarin were hydrolyzed by partially-fractionated enzymes of the beta-glucanolytic complex from Trichoderma harzianum Rifai. beta-Glucanase fractions (F-I and F-II) separated by gel permeation chromatography presented different modes of attack on botryosphaeran and laminarin. Botryosphaeran was hydrolyzed to the extent of 66% (F-I) and 98% (F-II) within 30 min, and its main hydrolysis products were gluco-oligosaccharides of DP >= 4, with lesser amounts of glucose, di- and tri-saccharides. The action of enzyme fractions I and II on laminarin resulted in 15% conversion to glucose, while the percentage of saccharification was radically different (70% for F-I and 25% for F-II). The different product arrays within the polysaccharide hydrolysates can be explained by the difference in the enzymes' specificities within each enzyme fraction, and the molecular structures of the polysaccharides and their complexity.en
dc.publisherWorld Scientific Publ Co Pte Ltd
dc.relation.ispartofCurrent Research Topics In Applied Microbiology and Microbial Biotechnology
dc.sourceWeb of Science
dc.subjectTrichoderma harzianum Rifaien
dc.subjectnon-cellulosic beta-glucanasesen
dc.titleEvaluation of the beta-glucanolytic enzyme complex of Trichoderma harzianum Rifai for the production of gluco-oligosaccharide fragments by enzymatic hydrolysis of 1,3;1,6-beta-D-glucansen
dc.typeTrabalho apresentado em evento
dcterms.rightsHolderWorld Scientific Publ Co Pte Ltd
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias Letras & Ciencias Exatas, São Paulo, Brazil
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
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