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dc.contributor.authorYamasaki, Lilian H. T. [UNESP]
dc.contributor.authorArcuri, Helen A.
dc.contributor.authorJardim, Ana Carolina G. [UNESP]
dc.contributor.authorBittar, Cintia [UNESP]
dc.contributor.authorde Carvalho-Mello, Isabel Maria V. G.
dc.contributor.authorRahal, Paula [UNESP]
dc.date.accessioned2014-05-20T14:01:17Z
dc.date.available2014-05-20T14:01:17Z
dc.date.issued2012-01-12
dc.identifierhttp://dx.doi.org/10.1186/1743-422X-9-14
dc.identifier.citationVirology Journal. London: Biomed Central Ltd., v. 9, p. 10, 2012.
dc.identifier.issn1743-422X
dc.identifier.urihttp://hdl.handle.net/11449/21652
dc.description.abstractBackground: HCV is prevalent throughout the world. It is a major cause of chronic liver disease. There is no effective vaccine and the most common therapy, based on Peginterferon, has a success rate of similar to 50%. The mechanisms underlying viral resistance have not been elucidated but it has been suggested that both host and virus contribute to therapy outcome. Non-structural 5A (NS5A) protein, a critical virus component, is involved in cellular and viral processes.Methods: The present study analyzed structural and functional features of 345 sequences of HCV-NS5A genotypes 1 or 3, using in silico tools.Results: There was residue type composition and secondary structure differences between the genotypes. In addition, second structural variance were statistical different for each response group in genotype 3. A motif search indicated conserved glycosylation, phosphorylation and myristoylation sites that could be important in structural stabilization and function. Furthermore, a highly conserved integrin ligation site was identified, and could be linked to nuclear forms of NS5A. ProtFun indicated NS5A to have diverse enzymatic and nonenzymatic activities, participating in a great range of cell functions, with statistical difference between genotypes.Conclusion: This study presents new insights into the HCV-NS5A. It is the first study that using bioinformatics tools, suggests differences between genotypes and response to therapy that can be related to NS5A protein features. Therefore, it emphasizes the importance of using bioinformatics tools in viral studies. Data acquired herein will aid in clarifying the structure/function of this protein and in the development of antiviral agents.en
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent10
dc.language.isoeng
dc.publisherBiomed Central Ltd.
dc.relation.ispartofVirology Journal
dc.sourceWeb of Science
dc.subjectHepatitis C virusen
dc.subjectNon-structural 5A proteinen
dc.subjectBioinformaticsen
dc.subjectGenotypeen
dc.subjectQuasispeciesen
dc.subjectIFN responseen
dc.titleNew insights regarding HCV-NS5A structure/function and indication of genotypic differencesen
dc.typeArtigo
dcterms.licensehttp://www.biomedcentral.com/about/license
dcterms.rightsHolderBiomed Central Ltd.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionLab Estudos Genom
dc.description.affiliationSão Paulo State Univ UNESP, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationSão Paulo Univ USP, Dept Clin Med, São Paulo, Brazil
dc.description.affiliationInstituto Butantan, Viral Immunol Lab, São Paulo, Brazil
dc.description.affiliationLab Estudos Genom, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ UNESP, Dept Biol, Sao Jose do Rio Preto, SP, Brazil
dc.identifier.doi10.1186/1743-422X-9-14
dc.identifier.wosWOS:000304652900001
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.fileWOS000304652900001.pdf
dc.identifier.lattes7991082362671212
unesp.author.lattes7991082362671212
dc.relation.ispartofjcr2.465
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