Estudo fitoquímico dos frutos de Pterogyne nitens
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Data
2021-02-10
Autores
Payão, Gabriela Migliatti
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Universidade Estadual Paulista (Unesp)
Resumo
A espécie arbórea Pterogyne nitens Tulasne (Fabaceae-Caesalpinioideae) tem demonstrado, através de diversos estudos, uma ampla série de substâncias bioativas como, por exemplo, flavonoides, terpenos, ácidos fenólicos e alcaloides guanidínicos. A partir dos frutos de P. nitens, foram preparados três diferentes extratos, o extrato hexânico (EHF), o acetônico (EAcF) e o etanólico (EEtF), sendo este, o alvo para continuidade do estudo cromatográfico. A purificação do extrato etanólico através de métodos cromatográficos forneceu duas substâncias isoladas: pteroginidina (6) e afzelina (18), as quais foram confirmadas através da análise de Ressonância Magnética Nuclear de Hidrogênio e Carbono Treze – RMN para 1H e 13C. Foi investigada a atividade antiparasitária in vitro contra Schistosoma mansoni linhagem LE dos três extratos por meio de testes de redução da viabilidade dos vermes adultos. A avaliação in vitro consistiu na observação de alterações fenotípicas classificadas em uma escala de viabilidade após períodos de 24, 48 e 72h de incubação com cada extrato. Os resultados foram expressos em porcentagem de redução da viabilidade. Nenhum dos três extratos demonstrou redução significativa da viabilidade. Os extratos também tiveram sua atividade tripanocida determinada contra Trypanosoma cruzi através do ensaio colorimétrico com MTT [3-(4,5-dimetiltiazol-2-yl)-2,5-difenil brometo de tetrazolina]. Os resultados foram expressos em porcentagem de morte de T cruzi. Todos os extratos foram classificados como não ativos devido à ausência de morte do parasito.
The tree species Pterogyne nitens Tulasne (Fabaceae-Caesalpinioideae) has demonstrated, through several studies, a wide range of bioactive substances such as flavonoids, terpenes, phenolic acids and guanidine alkaloids. From the fruits of P. nitens, three different extracts were prepared, hexanic (EHF), acetone (EAcF) and ethanolic (EEtF), this being the target for further study. The purification of the ethanolic extract using chromatographic methods provided three isolated substances: pterogynidine (6) e afzelin (18), which were confirmed by 1H and 13C Nuclear Magnetic Resonance (NMR). The in vitro antiparasitic activity against Schistosoma mansoni lineage LE was investigated for the three extracts using the adult worms viability reduction test. The in vitro evaluation consisted of observing phenotypic changes classified in a viability scale after periods of 24, 48 and 72 hours of incubation with each extract. The results were expressed as a percentage of reduced viability. None of the three extracts showed a significant reduction in viability. The extracts were also tested for trypanocidal activity against Trypanosoma cruzi through the colorimetric assay with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. The results were expressed as a percentage of T cruzi death. Both extracts were classified as non-active due to the absence of parasite death
The tree species Pterogyne nitens Tulasne (Fabaceae-Caesalpinioideae) has demonstrated, through several studies, a wide range of bioactive substances such as flavonoids, terpenes, phenolic acids and guanidine alkaloids. From the fruits of P. nitens, three different extracts were prepared, hexanic (EHF), acetone (EAcF) and ethanolic (EEtF), this being the target for further study. The purification of the ethanolic extract using chromatographic methods provided three isolated substances: pterogynidine (6) e afzelin (18), which were confirmed by 1H and 13C Nuclear Magnetic Resonance (NMR). The in vitro antiparasitic activity against Schistosoma mansoni lineage LE was investigated for the three extracts using the adult worms viability reduction test. The in vitro evaluation consisted of observing phenotypic changes classified in a viability scale after periods of 24, 48 and 72 hours of incubation with each extract. The results were expressed as a percentage of reduced viability. None of the three extracts showed a significant reduction in viability. The extracts were also tested for trypanocidal activity against Trypanosoma cruzi through the colorimetric assay with MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide]. The results were expressed as a percentage of T cruzi death. Both extracts were classified as non-active due to the absence of parasite death
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Palavras-chave
Pterogyne nitens, Pteroginidina, Alcaloides, Guanidina, Afzelina, Alkaloid, Guanidine, Pterogynidine