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dc.contributor.authordos Santos Cabrera, Marcia Perez [UNESP]
dc.contributor.authorArcisio-Miranda, Manoel
dc.contributor.authorda Costa, Laiana Cristina [UNESP]
dc.contributor.authorde Souza, Bibiana Monson [UNESP]
dc.contributor.authorBroggio Costa, Sabrina Thais [UNESP]
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorRuggiero Neto, Joao [UNESP]
dc.contributor.authorProcopio, Joaquim
dc.identifier.citationArchives of Biochemistry and Biophysics. New York: Elsevier B.V., v. 486, n. 1, p. 1-11, 2009.
dc.description.abstractIn the last decade, there has been renewed interest in biologically active peptides in fields like allergy, autoimmume diseases and antibiotic therapy. Mast cell degranulating peptides mimic G-protein receptors, showing different activity levels even among homologous peptides. Another important feature is their ability to interact directly with membrane phospholipids, in a fast and concentration-dependent way. The mechanism of action of peptide HR1 on model membranes was investigated comparatively to other mast cell degranulating peptides (Mastoparan, Eumenitin and Anoplin) to evidence the features that modulate their selectivity. Using vesicle leakage, single-channel recordings and zeta-potential measurements, we demonstrated that HR1 preferentially binds to anionic bilayers, accumulates, folds, and at very low concentrations, is able to insert and create membrane spanning ion-selective pores. We discuss the ion selectivity character of the pores based on the neutralization or screening of the peptides charges by the bilayer head group charges or dipoles. (C) 2009 Elsevier B.V. All rights reserved.en
dc.description.sponsorshipWinEDR package
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.publisherElsevier B.V.
dc.relation.ispartofArchives of Biochemistry and Biophysics
dc.sourceWeb of Science
dc.subjectMastoparan peptide HR1en
dc.subjectPeptide-membrane interactionen
dc.subjectIon channel-like activityen
dc.subjectMast cell degranulating peptidesen
dc.subjectLytic activityen
dc.subjectIonic selectivityen
dc.subjectPore activityen
dc.titleInteractions of mast cell degranulating peptides with model membranes: A comparative biophysical studyen
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationSão Paulo State Univ, UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Physiol & Biophys, Inst Biomed Sci, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Ctr Studies Social Insects, Inst Biosci, Rio Claro, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, IBILCE, Dept Phys, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Ctr Studies Social Insects, Inst Biosci, Rio Claro, SP, Brazil
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdWinEDR: 154550/2006-0
dc.description.sponsorshipIdCNPq: 142566/2005-5
dc.description.sponsorshipIdCAPES: 301064/2004-0
dc.description.sponsorshipIdCAPES: 310559/2006-5
dc.description.sponsorshipIdFAPESP: 06/57122-7
dc.description.sponsorshipIdFAPESP: 07/09657-0
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
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