Show simple item record

dc.contributor.authorJohansson, Hans-Olof
dc.contributor.authorMagaldi, Flavio Musa
dc.contributor.authorFeitosa, Eloi [UNESP]
dc.contributor.authorPessoa, Adalberto
dc.date.accessioned2014-05-20T14:02:38Z
dc.date.available2014-05-20T14:02:38Z
dc.date.issued2008-01-12
dc.identifierhttp://dx.doi.org/10.1016/j.chroma.2007.11.071
dc.identifier.citationJournal of Chromatography A. Amsterdam: Elsevier B.V., v. 1178, n. 1-2, p. 145-153, 2008.
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/11449/22084
dc.description.abstractThe partition of hemoglobin, lysozyme and glucose-6-phospate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na2SO4, pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively. (c) 2007 Elsevier B.V. All rights reserved.en
dc.format.extent145-153
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography A
dc.sourceWeb of Science
dc.subjectHemoglobinen
dc.subjectlysozymeen
dc.subjectGlucose-6-phosphate dehydrogenaseen
dc.subjectPolyethylene glycolen
dc.subjectsodium poly(acrylic acid)en
dc.subjectAqueous two-phase systemsen
dc.subjectLiquid-liquid extractionen
dc.titleProtein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systemsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv São Paulo, Dept Pharmaceut Technol & Biochem, BR-05508000 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Phys, Sao Jose do Rio Preto, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Phys, Sao Jose do Rio Preto, Brazil
dc.identifier.doi10.1016/j.chroma.2007.11.071
dc.identifier.wosWOS:000252477400017
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.relation.ispartofjcr3.716
dc.relation.ispartofsjr1,378
Localize o texto completo

Files in this item

FilesSizeFormatView

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record