Evidence supporting the recognition of Cole latent virus as a distinct carlavirus
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Data
2002-06-01
Autores
Belintani, P.
Gaspar, J. O.
Targon, MLPN
Machado, M. A.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-Blackwell
Resumo
Some molecular properties are described of Cole latent virus (CoLV), hitherto designated a tentative species of the Carlavirus genus. CoLV genomic RNA (Ribonucleic acid) of 8.3 Kb is polyadenylated. Two unencapsidated polyadenylated subgenomic RNAs (2.6 and 1.3 Kb) and three double-stranded RNAs (dsRNAs) (8.3, 2.6 and 1.3 Kbp), which are twice the size of the genomic and subgenomics ssRNAs, are produced in CoLV-infected plants, two additional dsRNAs (7.2 and 6.3 Kbp) were also detected plant extracts. By using a Carlavirus specific primer and a CoLV cDNA, a-3'-terminus fragment of 116 bp was amplified; it had homology with the carlaviruses Potato virus M (62%)., Hop latent virus (37%) and Blueberry scorch virus (36%) but no significant homology with 11 other carlaviruses. These results support the classification of CoLV as a distinct species of the Carlavirus genus.
Descrição
Palavras-chave
Brassica oleracea var. acephala, polyadenylation, genomic and subgenomic RNAs, carlavirus, Brasil
Como citar
Journal of Phytopathology. Hoboken: Wiley-blackwell, v. 150, n. 6, p. 330-333, 2002.