Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)
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A RNase of Aspergillus flavlpes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55°C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3′-GMP from yeast RNA.
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Gomes, Eleni; Serzedello, Alcides; Da Silva, Roberto. Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501). Microbios, v. 96, n. 383, p. 39-50, 1998. Available at: <http://hdl.handle.net/11449/224099>.