A degenerate primer allows amplification of part of the 3 '-terminus of three distinct carlavirus species
Nenhuma Miniatura disponível
Data
2008-03-01
Autores
Gaspar, J. O. [UNESP]
Belintani, P. [UNESP]
Almeida, A. M. R.
Kitajima, E. W.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Sequences of the coat protein amino acids of definitive and tentative species of carlaviruses deposited in GenBank were aligned and a region of seven amino acids (GLGVPTE) was found to be conserved. The corresponding nucleotides were aligned, allowing the design of a degenerate primer that together with an oligo dT anti-sense primer, was effective for the detection of three distinct carlavirus species, two transmitted by aphids and one by whitefly. These primers have the advantage that about 940 nt from the 3'-terminus, comprising part of the CP gene (about 60%), the 11 K gene, and the terminal untranslated region can be amplified for sequencing. The fact that this amino acid sequence is conserved in almost all of the sequenced carlaviruses, allows the prediction that this primer pair will be useful as a diagnostic tool for carlavirus species. (C) 2007 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
carlavirus identification, diagnosis, Flexiviridae, RT-PCR
Como citar
Journal of Virological Methods. Amsterdam: Elsevier B.V., v. 148, n. 1-2, p. 283-285, 2008.