Molecular identification of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila
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Data
2012-01-01
Autores
Mota, Adolfo Jose
Back Brito, Graziella Nuernberg [UNESP]
Nobrega, Francisco G. [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Genética
Resumo
Traditional phenotypic methods and commercial kits based on carbohydrate assimilation patterns are unable to consistently distinguish among isolates of Pichia guilliermondii, Debaryomyces hansenii and Candida palmioleophila. As result, these species are often misidentified. In this work, we established a reliable method for the identification/differentiation of these species. Our assay was validated by DNA sequencing of the polymorphic region used in a real-time PCR assay driven by species-specific probes targeted to the fungal ITS 1 region. This assay provides a new tool for pathogen identification and for epidemiological, drug resistance and virulence studies of these organisms.
Descrição
Palavras-chave
Candida palmioleophila, Debaryomyces hansenii, differential identification, Pichia guilliermondii, real-time PCR
Como citar
Genetics and Molecular Biology. Sociedade Brasileira de Genética, v. 35, n. 1, p. 122-125, 2012.