In vivo toxicogenic potential of Salix alba (Salicaceae) bark extract
Nenhuma Miniatura disponível
Data
2022-01-01
Autores
Maistro, Edson Luis [UNESP]
Terrazzas, Peterson Menezes [UNESP]
Sawaya, Alexandra Christinie Helena Frankland
Rosa, Paulo Cesar Pires
Perazzo, Fábio Ferreira
de Mascarenhas Gaivão, Isabel O’Neill
Título da Revista
ISSN da Revista
Título de Volume
Editor
Resumo
Salix alba (white willow) bark extract is widely used for conditions associated with inflammation, fever, microbial infection or pain. Exposure of human cultured leukocytes to S. alba in vitro noted a genotoxic response. However, data regarding the influence of this bark extract on DNA damage in vivo are lacking. The main goal of this study was to examine the potential of S.alba bark extract to induce DNA damage and chromosome aberrations in an in vivo model using cells obtained from male Swiss albino mice administered the compound orally. The extract was administered by oral gavage daily for 7 days at doses of 500, 1000, or 2000 mg/kg b.w. Genotoxicity analysis was performed using the comet assay on peripheral blood leukocytes, as well as liver, bone marrow, heart, and testicular cells collected 4 hr after the last treatment and the micronucleus (MN) test on bone marrow cells. In essence cells were collected 28 hr after the penultimate treatment Data demonstrated that S. alba bark extract did not induce significant DNA damage in any cell types examined, or clastogenic/aneugenic effects as detected by the MN test at the three tested doses. Under these experimental conditions, evidence indicates that S.alba bark extract did not initiate genotoxic or chromosome aberrations in various mouse cells investigated.
Descrição
Palavras-chave
Salicaceae genotoxicity, Salix comet assay, Salix micronucleus test, White willow
Como citar
Journal of Toxicology and Environmental Health - Part A: Current Issues, v. 85, n. 3, p. 121-130, 2022.