Avaliação das propriedades físico-químicas de cimentos biocerâmicos em diferentes modelos experimentais
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Data
2022-05-23
Autores
Ferraz, Danilo Cassiano
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Editor
Universidade Estadual Paulista (Unesp)
Resumo
Modelos experimentais podem influenciar a avaliação de propriedades de materiais biocerâmicos. Este estudo avaliou o efeito de meios de imersão na análise das propriedades físico-químicas de materiais biocerâmicos, além de avaliar um novo cimento reparador e endodôntico. Publicação 1: Foram utilizados diferentes meios de imersão: água destilada, solução salina tamponada de fosfato (PBS) e método in vivo (fluido tissular do tecido subcutâneo de ratos). A alteração volumétrica dos cimentos reparadores Bio-C Repair (BCR, Angelus), Biodentine (BD, Septodont) e IRM (IRM, Dentsply Sirona) foi avaliada em microtomografia computadorizada (micro-CT). Tubos de PVC (n=7) foram preenchidos e escaneados em micro-CT, em seguida imersos em 7,5 mL de água destilada, PBS ou fluido tissular do tecido subcutâneo de ratos por 7 dias. IRM apresentou maior alteração volumétrica que BCR e BIO para todos os meios de imersão. BIO e BCR apresentaram alteração volumétrica similar quando imersos em PBS e água destilada. No meio in vivo, BIO e BCR apresentaram menor alteração volumétrica, além de um aumento volumétrico para BCR. Portanto, o meio de imersão pode influenciar a alteração volumétrica de cimentos biocerâmicos. Além disso, cimentos reparadores apresentam maior estabilidade volumétrica quando avaliados em método in vivo. Publicação 2: Avaliou as propriedades físico-químicas de alteração volumétrica, solubilidade e escoamento do cimento a base de silicato de cálcio híbrido NeoMTA 2 (Avalon Biomed) na proporção reparadora (NMTA2R) e obturadora (NMTA2S) comparado a BioRoot RCS (BIR, Septodont) e MTA Repair HP (MTAHP, Angelus). O escoamento foi realizado conforme normas ISO 6876 e a radiopacidade foi avaliada por análise radiográfica em milímetros de alumínio. Para a análise volumétrica em micro-CT, tubos de dentina (4 mm altura x 1,5 mm de diâmetro, n=5) foram preenchidos com os materiais testados. As amostras foram escaneadas após presa e após imersão em água destilada ou PBS por 7 dias. O escoamento foi melhor para NMTA2S que para BIR. NMTA2R apresentou a maior radiopacidade entre todos os materiais testados. Todos os materiais apresentaram radiopacidade e escoamento de acordo com ISO 6876. Os cimentos apresentaram alteração volumétrica diferente entre si. BIR resultou em ganho de volume, com maior aumento em PBS. NMTA2S, NMTA2R e MTAHP apresentou perda de volume. NMTA2S apresentou a maior alteração volumétrica, porém abaixo de 1%. Conclui-se NeoMTA 2 reparador e endodôntico apresentam radiopacidade de acordo com a ISO 6876. NeoMTA 2S apresentou escoamento adequado. NeoMTA 2 reparador e endodôntico apresentaram perda volumétrica abaixo de 1%, com propriedades compatíveis com a aplicação clínica.
Experimental models can influence the evaluation of properties of bioceramic materials. This study evaluated the effect of immersion solutions on the analysis of the physicochemical properties of bioceramic materials, in addition to evaluating a new reparative and endodontic material. Publication 1: Different immersion solutions were used: distilled water, phosphate-buffered saline (PBS) and tissue fluid from the subcutaneous tissue of rats. The volumetric change in micro-CT of the repair cements Bio-C Repair (BCR, Angelus), Biodentine (BD, Septodont) compared to IRM (IRM, Dentsply Sirona) was evaluated. PVC tubes (n=7) were filled and scanned in micro-CT, then immersed in 7.5 mL of distilled water, PBS or tissue fluid from the subcutaneous tissue of rats for 7 days. IRM showed higher volumetric change than BCR and BIO in all soaking solutions. BIO and BCR presented similar volumetric change when immersed in PBS and distilled water. When immersed in vivo tissue fluid, BIO and BCR showed lower volumetric change, including an increase in volume for BCR. In conclusion, the soaking media influence the evaluation of the volumetric change of calcium silicate repair materials. Calcium silicate cements present greater volumetric stability when in vivo method was used. Publication 2: Were evaluated the physicochemical properties of volumetric change, solubility and flow of a hybrid calcium silicate based material: NeoMTA 2 (Avalon Biomed) in the repair (NMTA2R) and endodontic sealer (NMTA2S) consistencies compared to BioRoot RCS (BIR, Septodont) and MTA Repair HP (MTAHP, Angelus). Flow was evaluated based on ISO 6876 standard and radiopacity was evaluated by radiographic analysis in millimeters of aluminum. Dentin tubes 4 mm high by 1.5 mm in diameter were filled with the materials for volumetric analysis in micro-CT. Samples were scanned after materials setting and after immersion in distilled water or PBS for 7 days. Flow was greater for NMTA2S than BIR. NMTA2R had the highest radiopacity among all materials. All materials presented flow and radiopacity according to ISO 6876 standard. Materials presented different volumetric change. BIR showed volume increase, with a greater increase in PBS. NMTA2S, NMTA2R and MTAHP presented volumetric decrease. NMTA2S had the higher volume change, but lower than 1%. NeoMTA 2 Sealer and Repair presented radiopacity according to ISO 6876. NeoMTA 2 Sealer showed flow higher than BioRoot. NMTA2 Sealer and NMTA2 Repair showed a volumetric decrease below 1%, with properties compatible with clinical application.
Experimental models can influence the evaluation of properties of bioceramic materials. This study evaluated the effect of immersion solutions on the analysis of the physicochemical properties of bioceramic materials, in addition to evaluating a new reparative and endodontic material. Publication 1: Different immersion solutions were used: distilled water, phosphate-buffered saline (PBS) and tissue fluid from the subcutaneous tissue of rats. The volumetric change in micro-CT of the repair cements Bio-C Repair (BCR, Angelus), Biodentine (BD, Septodont) compared to IRM (IRM, Dentsply Sirona) was evaluated. PVC tubes (n=7) were filled and scanned in micro-CT, then immersed in 7.5 mL of distilled water, PBS or tissue fluid from the subcutaneous tissue of rats for 7 days. IRM showed higher volumetric change than BCR and BIO in all soaking solutions. BIO and BCR presented similar volumetric change when immersed in PBS and distilled water. When immersed in vivo tissue fluid, BIO and BCR showed lower volumetric change, including an increase in volume for BCR. In conclusion, the soaking media influence the evaluation of the volumetric change of calcium silicate repair materials. Calcium silicate cements present greater volumetric stability when in vivo method was used. Publication 2: Were evaluated the physicochemical properties of volumetric change, solubility and flow of a hybrid calcium silicate based material: NeoMTA 2 (Avalon Biomed) in the repair (NMTA2R) and endodontic sealer (NMTA2S) consistencies compared to BioRoot RCS (BIR, Septodont) and MTA Repair HP (MTAHP, Angelus). Flow was evaluated based on ISO 6876 standard and radiopacity was evaluated by radiographic analysis in millimeters of aluminum. Dentin tubes 4 mm high by 1.5 mm in diameter were filled with the materials for volumetric analysis in micro-CT. Samples were scanned after materials setting and after immersion in distilled water or PBS for 7 days. Flow was greater for NMTA2S than BIR. NMTA2R had the highest radiopacity among all materials. All materials presented flow and radiopacity according to ISO 6876 standard. Materials presented different volumetric change. BIR showed volume increase, with a greater increase in PBS. NMTA2S, NMTA2R and MTAHP presented volumetric decrease. NMTA2S had the higher volume change, but lower than 1%. NeoMTA 2 Sealer and Repair presented radiopacity according to ISO 6876. NeoMTA 2 Sealer showed flow higher than BioRoot. NMTA2 Sealer and NMTA2 Repair showed a volumetric decrease below 1%, with properties compatible with clinical application.
Descrição
Palavras-chave
Endodontia, Microtomografia por Raio-X, Propriedades físicas, Endodontics, X-Ray Microtomography, Physical Properties