Comparação da atividade osteopromotora de membranas nacionais de pericárdio bovino no processo de regeneração óssea guiada
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Data
2023-03-03
Autores
Ferriolli, Stéfani Caroline [UNESP]
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Universidade Estadual Paulista (Unesp)
Resumo
O êxito de membranas absorvíveis já é descrito na literatura, porém, sua vulnerabilidade à degradação do colágeno e a estabilidade dimensional reduzida ainda são fatores deliberativos em pesquisas científicas [1-2] tornando pertinente a busca por materiais capazes de suprir essas limitações, bem como também cumprir as exigências para adequada reconstrução físico biológica dos defeitos ósseos. Nesse contexto, as membranas colágenas de pericárdio bovino têm como premissa retardar esse prejuízo e aumentar a resistência mecânica [3-4]. O objetivo deste trabalho é avaliar o potencial osteopromotor das membranas nacionais de pericárdio bovino recentemente inseridas no mercado, em comparação com a membrana de derme suína amplamente utilizada Bio-Gide® (Geistlich Wohlhusen, Suíça) implantando-as em defeitos críticos criados em calvária de ratos. Foram utilizados 96 ratos Albinus Wistar divididos em 4 grupos, n=: grupo (GC-) controle negativo somente com coágulo; grupo (GT1) que recebeu a membrana de pericárdio bovino (Surgitime Collagen® - Bioinnovation); grupo (GT2): membrana de pericárdio bovino (Techgraft - Baumer®); e grupo controle positivo (GT+): membrana de derme porcina (Bio-Gide®). Cada grupo foi dividido em 04 subgrupos, de acordo com os tempos de eutanásia: 7, 15, 30 e 60 dias. Após a eutanásia, as peças foram processadas e os trâmites laboratoriais realizados para avaliação das análises histomorfométrica, perfil inflamatório e microtomografica. Os resultados quantitativos e qualitativos obtidos demonstram que as membranas de pericárdio bovino tiveram bom comportamento biológico, promoveram neoformação óssea, e auxiliaram no processo de regeneração óssea guiada, apresentando comportamento biológico semelhante a membrana de derme suína e superior ao grupo controle negativo, fazendo das membranas nacionais Surgitime Collagen® e Techgraft uma alternativa pertinente a membrana de derme suína Bio-Gide® amplamente utilizada nos processos de regeneração óssea guiada.
The success of absorbable membranes is already described in the literature, however, their vulnerability to collagen degradation and reduced dimensional stability are still deliberative factors in scientific research [1,2] making relevant the search for materials capable of meeting these limitations, as well as meeting the requirements for adequate biological physical reconstruction of bone defects. In this context, bovine pericardium collagen membranes have the premise of delaying this injury and increasing mechanical resistance [3,4]. The objective of this work is to evaluate the osteopromoter potential of bovine pericardium reticulated national membranes recently inserted on the market, in comparison with a widely used non-reticulated pig collagen membrane, Bio-Gide® (Geistlich Wohlhusen, Switzerland) implanting them in critical defects created in rat calvary. Ninety-six Albinus Wistar rats were divided into 4 groups, n=6: group (GC-) negative control only with clot; group (GT1) that received bovine pericardium membrane (Surgitime Collagen® - Bioinnovation); group (GT2): bovine pericardium membrane (Techgraft - Baumer®); and positive control group (GT+): porcin dermis membrane (Bio-Gide®). Each group was divided into 04 subgroups, according to euthanasia times: 7, 15, 30 and 60 days. After euthanasia, the parts were processed and laboratory procedures performed to evaluate histomorphometric, inflammatory profile and microtomographic analyses. The quantitative and qualitative results obtained demonstrate that bovine pericardium membranes had good biological behavior, promoted bone neoformation, and assisted in the guided bone regeneration process, presenting behavior similar to the swine dermis membrane and superior to the control group, making the national membranes Surgitime Collagen® and Techgraft a relevant alternative to bio-gide swine dermis membrane® widely used in guided bone regeneration processes.
The success of absorbable membranes is already described in the literature, however, their vulnerability to collagen degradation and reduced dimensional stability are still deliberative factors in scientific research [1,2] making relevant the search for materials capable of meeting these limitations, as well as meeting the requirements for adequate biological physical reconstruction of bone defects. In this context, bovine pericardium collagen membranes have the premise of delaying this injury and increasing mechanical resistance [3,4]. The objective of this work is to evaluate the osteopromoter potential of bovine pericardium reticulated national membranes recently inserted on the market, in comparison with a widely used non-reticulated pig collagen membrane, Bio-Gide® (Geistlich Wohlhusen, Switzerland) implanting them in critical defects created in rat calvary. Ninety-six Albinus Wistar rats were divided into 4 groups, n=6: group (GC-) negative control only with clot; group (GT1) that received bovine pericardium membrane (Surgitime Collagen® - Bioinnovation); group (GT2): bovine pericardium membrane (Techgraft - Baumer®); and positive control group (GT+): porcin dermis membrane (Bio-Gide®). Each group was divided into 04 subgroups, according to euthanasia times: 7, 15, 30 and 60 days. After euthanasia, the parts were processed and laboratory procedures performed to evaluate histomorphometric, inflammatory profile and microtomographic analyses. The quantitative and qualitative results obtained demonstrate that bovine pericardium membranes had good biological behavior, promoted bone neoformation, and assisted in the guided bone regeneration process, presenting behavior similar to the swine dermis membrane and superior to the control group, making the national membranes Surgitime Collagen® and Techgraft a relevant alternative to bio-gide swine dermis membrane® widely used in guided bone regeneration processes.
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Palavras-chave
Regeneração óssea, Materiais biomédicos, Ossos, Membranas, Bone regeneration