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dc.contributor.authorApparicio, M. [UNESP]
dc.contributor.authorFerreira, C. R.
dc.contributor.authorTata, A.
dc.contributor.authorSantos, V. G.
dc.contributor.authorAlves, A. E.
dc.contributor.authorMostachio, G. Q. [UNESP]
dc.contributor.authorPires-Butler, E. A. [UNESP]
dc.contributor.authorMotheo, T. F.
dc.contributor.authorPadilha, L. C. [UNESP]
dc.contributor.authorPilau, E. J.
dc.contributor.authorGozzo, F. C.
dc.contributor.authorEberlin, M. N.
dc.contributor.authorLo Turco, E. G.
dc.contributor.authorLuvoni, G. C.
dc.contributor.authorVicente, W. R R [UNESP]
dc.date.accessioned2014-05-20T13:15:22Z
dc.date.available2014-05-20T13:15:22Z
dc.date.issued2012-12-01
dc.identifierhttp://dx.doi.org/10.1111/rda.12003
dc.identifier.citationReproduction In Domestic Animals. Hoboken: Wiley-blackwell, v. 47, p. 113-117, 2012.
dc.identifier.issn0936-6768
dc.identifier.urihttp://hdl.handle.net/11449/2529
dc.description.abstractContents The aim of the present study was to investigate the level of information on the chemical structures and relative abundances of lipids present in cat and dog oocytes by matrix-assisted laser desorption mass spectrometry (MALDI-MS). The MALDI-MS approach requires a simple analysis workflow (no lipid extraction) and few samples (two or three oocytes per analysis in this work) providing concomitant profiles of both intact phospholipids such as sphingomyelins (SM) and phosphatidylcholines (PC) as well as triacylglycerols (TAG). The lipids were detected in oocytes by MALDI using dihydroxybenzoic acid (DHB) as the matrix. The most abundant lipid present in the MS profiles of bitch and queen oocytes was a PC containing 34 carbons and one unsaturation [PC (34:1)]. Oocytes of these two species are characterized by differences in PC and TAG profiles detected qualitatively as well as by means of principal component analysis (PCA). Cat oocytes were mainly discriminated by more intense C52 and C54 TAG species and a higher number of unsaturations, indicating predominantly linoleic and oleic fatty acyl residues. Comparison of the lipid profile of bitch and queen oocytes with that of bovine oocytes revealed some similarities and also some species specificity: TAG species present in bovine oocytes were also present in bitches and queens; however, a more pronounced contribution of palmitic, stearic and oleic fatty acid residues was noticed in the lipid profile of bovine oocytes. MALDI-MS provides novel information on chemical lipid composition in canine and feline oocytes, offering a suitable tool to concomitantly monitor, in a nearly direct and simple fashion the composition of phospholipids and TAG. This detailed information is highly needed to the development of improved protocols for in vitro culture and cryopreservation of cat and dog oocytes.en
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent113-117
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofReproduction in Domestic Animals
dc.sourceWeb of Science
dc.titleChemical Composition of Lipids Present in Cat and Dog Oocyte by Matrix-Assisted Desorption Ionization Mass Spectrometry (MALDI- MS)en
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Milan
dc.description.affiliationUniv Estadual Paulista, UNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Chem, ThomSon Mass Spectrometry Lab, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Chem, Dalton Mass Spectrometry Lab, Campinas, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Dept Surg, Div Urol, Human Reprod Sect, São Paulo, Brazil
dc.description.affiliationUniv Milan, Dipartimento Sci Vet Salute Prod Anim & Sicurezza, Milan, Italy
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, FCAV, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP, Brazil
dc.identifier.doi10.1111/rda.12003
dc.identifier.wosWOS:000312888300025
dc.rights.accessRightsAcesso restrito
dc.description.sponsorshipIdCNPq: 150184/2011-5
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
dc.relation.ispartofjcr1.422
dc.relation.ispartofsjr0,594
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