Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs
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Data
2008-01-01
Autores
Uliana, Carolina V. [UNESP]
Riccardi, Carla S. [UNESP]
Tognolli, Joao O. [UNESP]
Yamanaka, Hideko [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Brasileira Quimica
Resumo
Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL(-1) and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1: 1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.
Descrição
Palavras-chave
biosensor, hepatitis C virus, fractional factorial design
Como citar
Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 19, n. 4, p. 782-787, 2008.