Detection of DNA Nucleotides on Pretreated Boron Doped Diamond Electrodes
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Data
2011-01-01
Autores
Garbellini, Gustavo S. [UNESP]
Uliana, Carolina V. [UNESP]
Yamanaka, Hideko [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Soc Brasileira Quimica
Resumo
The individual detection and equimolar mixture of DNA nucleotides guanosine monophosphate (GMP), adenosine monophosphate (AMP), thymidine (TMP) and cytidine (CMP) 5'-monophosphate using square wave voltammetry was performed on boron doped diamond (BDD) electrodes cathodically (Red-DDB) and anodically (Oxi-DDB) pretreated. The oxidation of individual DNA nucleotides was more sensitive on Oxi-BDD electrode. In a simultaneous detection of nucleotides, the responses of GMP, AMP, TMP and CMP were very adequate on both treated electrodes. Particularly, more sensitive and separate peaks for TMP and CMP on Oxi-BDD and Red-BDD electrodes, respectively, were observed after deconvolution procedure. The detection of nucleotides in aqueous solutions will certainly contribute for genotoxic evaluation of substances and hybridization reactions by immobilizing ss or ds-DNA on BDD surface.
Descrição
Palavras-chave
DNA, nucleotides, diamond electrodes, surface termination
Como citar
Journal of The Brazilian Chemical Society. São Paulo: Soc Brasileira Quimica, v. 22, n. 7, p. 1241-1245, 2011.