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dc.contributor.authorJosé, Anderson Cleiton
dc.contributor.authorSilva, Edvaldo Aparecido Amaral da [UNESP][
dc.contributor.authorDavide, Antonio Claudio
dc.contributor.authorToorop, Peter
dc.date.accessioned2014-05-20T15:07:34Z
dc.date.available2014-05-20T15:07:34Z
dc.date.issued2011-06-01
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132011000300006
dc.identifier.citationBrazilian Archives of Biology and Technology. Brazilian Archives of Biology and Technology, v. 54, n. 3, p. 465-476, 2011.
dc.identifier.issn1516-8913
dc.identifier.urihttp://hdl.handle.net/11449/26576
dc.description.abstractThe effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which 21 showed differential expression, correlated with desiccation and germination, by increased or decreased expression. After MS/MS sequencing, three protein spots produced spectra that matched to a Magnolia salicifolia legumin precursor. Results suggested an involvement of this protein in the events taking place during the drying and subsequent imbibitions of the dried seeds.en
dc.description.sponsorshipRoyal Botanic Gardens, Kew, UK
dc.description.sponsorshipMillennium Seed Bank (Kew)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent465-476
dc.language.isoeng
dc.publisherBrazilian Archives of Biology and Technology
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.sourceSciELO
dc.subjectdesiccation toleranceen
dc.subjectforest seeden
dc.subjectleguminen
dc.subjectproteomicsen
dc.titleProtein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seedsen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/babt/paboutj.htm
dc.contributor.institutionUniversidade Federal de Lavras (UFLA)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionRoyal Botanic Gardens Seed Conservation Department
dc.description.affiliationUniversidade Federal de Lavras (UFLA) Departamento de Ciências Florestais
dc.description.affiliationUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agronômicas de Botucatu
dc.description.affiliationRoyal Botanic Gardens Seed Conservation Department
dc.description.affiliationUnespUniversidade Estadual Paulista Júlio de Mesquita Filho Faculdade de Ciências Agronômicas de Botucatu
dc.identifier.doi10.1590/S1516-89132011000300006
dc.identifier.scieloS1516-89132011000300006
dc.identifier.wosWOS:000291849900006
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agronômicas, Botucatupt
dc.identifier.fileS1516-89132011000300006.pdf
dc.identifier.lattes9972643774491301
dc.identifier.orcid0000-0001-6454-1488
unesp.author.lattes9972643774491301[2]
unesp.author.orcid0000-0002-5642-8001[1]
unesp.author.orcid0000-0002-9328-9733[4]
unesp.author.orcid0000-0001-6454-1488[2]
dc.relation.ispartofjcr0.676
dc.relation.ispartofsjr0,281
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