Estudo da enxertia hipocotiledonar do maracujazeiro-amarelo sobre dois porta-enxertos, através de microscopia eletrônica de varredura
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Data
2010-06-01
Autores
Nogueira Filho, Geraldo Costa
Roncatto, Givanildo
Ruggiero, Carlos [UNESP]
Oliveira, João Carlos de [UNESP]
Malheiros, Euclides Braga [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira de Fruticultura
Resumo
O objetivo deste trabalho foi verificar quanto tempo após a realização da enxertia hipocotiledonar ocorre a soldadura ou adesão entre enxerto e porta-enxerto, e também a formação da ponte de calo, verificada pelo total preenchimento da fenda por tecido meristemático secundário (calo). Foram produzidas 56 mudas de maracujazeiro-amarelo (Passiflora edulis Sims f. flavicarpa Deg.) pela enxertia hipocotiledonar em fenda cheia no topo sobre dois porta-enxertos (P. edulis Sims f. flavicarpa Deg. e P. alata Dryander). Aos 0, 3, 6, 9, 12, 15 e 18 dias após a enxertia, coletou-se a região da enxertia de quatro mudas de cada combinação. O material coletado foi fixado em solução de glutaraldeído a 3%, pós-fixado em tetróxido de ósmio a 2%, desidratado em uma série de álcool etílico (30, 40, 50, 60, 70, 80, 90, 100%), levado ao secador de ponto crítico (CO2), montado, metalizado com ouro - paládio (35 nm) e, por fim, observados e eletromicrografados em microscópio eletrônico de varredura JEOL JSM 5410 (operado em 15 kV). Verificou-se que, aos seis dias após a enxertia, a soldadura para o porta-enxerto Passiflora alata já havia ocorrido, o que só foi constatado para Passiflora edulis f. flavicarpa aos nove dias. Também, aos nove dias, observou-se para ambos os porta-enxertos a completa formação da ponte de calo, indicando que, a partir daí, pode-se iniciar o processo de aclimatação da muda, para levá-la a um ambiente de menor umidade.
The objective of this work was to verify how much time after the accomplishment of the hypocotyledonary grafting occurs the weld or adhesion between graft and rootstock, and also the formation of the bridge of callus, verified for the total fulfilling of the slot for secondary meristematic tissue (callus). It was produced 56 seedlings of yellow passion fruit (P. edulis Sims f. flavicarpa Deg.) by the hypocotyledonary grafting in full crack in the top on two rootstocks (P. edulis Sims f. flavicarpa Deg. e P. alata Dryander). on the 0, 3, 6, 9, 12, 15 and 18 days after grafting, it was collected the region of the grafting of four seedlings of each combination. The collected material was fixed in a solution of glutaraldehyde at 3%, post-fixed in osmium tetroxide of the osmium at 2%, dehydrated in a series of ethyl alcohol (30, 40, 50, 60, 70, 80, 90, 100%), led to the drier of critical point (CO2), mounted, with metalized with gold - palladium (35 nm) and, finally, observed and eletromicrographed in a scanning electronic microscope JEOL JSM 5410 (operated at 15 kV). It was discovered that, six days after grafting, the welding for the rootstock P. alata had already occurred, which was only evidenced by P. edulis f. flavicarpa on the ninth days. Also, on the ninth day, it was observed on both rootstocks, the complete bridge of callus formation, indicating that, from this point, you can start the acclimation process of seedling, to take it to an environment of lower humidity.
The objective of this work was to verify how much time after the accomplishment of the hypocotyledonary grafting occurs the weld or adhesion between graft and rootstock, and also the formation of the bridge of callus, verified for the total fulfilling of the slot for secondary meristematic tissue (callus). It was produced 56 seedlings of yellow passion fruit (P. edulis Sims f. flavicarpa Deg.) by the hypocotyledonary grafting in full crack in the top on two rootstocks (P. edulis Sims f. flavicarpa Deg. e P. alata Dryander). on the 0, 3, 6, 9, 12, 15 and 18 days after grafting, it was collected the region of the grafting of four seedlings of each combination. The collected material was fixed in a solution of glutaraldehyde at 3%, post-fixed in osmium tetroxide of the osmium at 2%, dehydrated in a series of ethyl alcohol (30, 40, 50, 60, 70, 80, 90, 100%), led to the drier of critical point (CO2), mounted, with metalized with gold - palladium (35 nm) and, finally, observed and eletromicrographed in a scanning electronic microscope JEOL JSM 5410 (operated at 15 kV). It was discovered that, six days after grafting, the welding for the rootstock P. alata had already occurred, which was only evidenced by P. edulis f. flavicarpa on the ninth days. Also, on the ninth day, it was observed on both rootstocks, the complete bridge of callus formation, indicating that, from this point, you can start the acclimation process of seedling, to take it to an environment of lower humidity.
Descrição
Palavras-chave
Passiflora spp., sweet passion fruit plant, Vegetative propagation, compatibility, electromicrographic, Passiflora spp., maracujazeiro-doce, Propagação vegetativa, Compatibilidade, eletromicrografia
Como citar
Revista Brasileira de Fruticultura. Sociedade Brasileira de Fruticultura, v. 32, n. 2, p. 647-652, 2010.