Development and application of a Saccharomyces cerevisiae-expressed nucleocapsid protein-based enzyme-linked immunosorbent assay for detection of antibodies against infectious bronchitis virus
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2005-04-01
Autores
Gibertoni, A. M.
Montassier, MDS
Sena, JAD
Givisiez, PEN
Furuyama, CRAG
Montassier, H. J.
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Amer Soc Microbiology
Resumo
A Saccharomyces cerevisiae-expressed nucleocapsid (N) polypeptide of the M41 strain of infectious bronchitis virus (IBV) was used as antigen in a recombinant yeast-expressed N protein-based enzyme-linked immunosorbent assay (Y-N-ELISA). The Y-N-ELISA was rapid, sensitive, and specific for detecting chicken serum antibodies to IBV, and it compared favorably with a commercial ELISA.
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Journal of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 43, n. 4, p. 1982-1984, 2005.