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dc.contributor.authorMachado, Rosangela Z.
dc.contributor.authorLandim, Lorivaldo P.
dc.contributor.authorMineo, Tiago W. P.
dc.contributor.authorCarvalhoi, Ana F.
dc.contributor.authorGennari, Solange M.
dc.contributor.authorMiglino, Maria A.
dc.date.accessioned2014-05-20T13:15:50Z
dc.date.available2014-05-20T13:15:50Z
dc.date.issued2007-01-01
dc.identifierhttp://cbpv.com.br/rbpv/documentos/1612007/c16121_25.pdf
dc.identifier.citationRevista Brasileira de Parasitologia Veterinaria. São Paulo: Brazilian Coll Veterinary Parasitology, v. 16, n. 1, p. 21-25, 2007.
dc.identifier.issn0103-846X
dc.identifier.urihttp://hdl.handle.net/11449/2879
dc.description.abstractNeospora caninum is an aplicomplexan parasite that has brought several concerns to cattle raisers worldwide due to its relationship to fetal loss. However, the mechanism of the parasite's transplacental infection and induced abortions are not completely understood. Bovine trophoblastic binucleated cells (BNC) play a major role in the maternal-fetal interactions, migrating during the entire pregnancy from chorionic connections to uterine epithelium. This study aimed to investigate the possible role of BNC as phagocytic cells and its participation in the bovine transplacental infection of N. caninum. BNC was isolated by discontinuous Percoll gradient, and characterized by Hoeschst 33342 nucleus-specific staining. Isolated BNC were cultured in DMEM supplemented with 10% bovine fetal serum, and infected with 10(4) tachyzoites of N. caninum NC-1 strain. Parasite invasion was visualized by indirect immunofluorescence and Giemsa technique. Multiplication of parasites took place in 2-3 day cycles. Healthy cows' placenta and normal and infected cultured BNC was immunostained with monoclonal antibodies against CD-163, MAC-387 and NOS, demonstrating their phagocyte capacity. Thus, BNC was characterized as cells with macrophagic activity, which may host N. caninum in vitro. Therefore, we may conclude that BNC could potentially participate in the transplacental infection of bovine neosporosis.en
dc.format.extent21-25
dc.language.isoeng
dc.publisherBrazilian Coll Veterinary Parasitology
dc.relation.ispartofRevista Brasileira de Parasitologia Veterinária
dc.sourceWeb of Science
dc.subjectNeospora caninumpt
dc.subjectbovine trophoblastic binucleated cellspt
dc.subjectImmunohistochemistrypt
dc.subjectcell culturept
dc.titlePossible role of bovine trophoblast giant cells in transplacental transmission of Neospora caninum in cattleen
dc.typeArtigo
dcterms.licensehttp://www.scielo.br/revistas/rbpv/paboutj.htm
dcterms.rightsHolderBrazilian Coll Veterinary Parasitology
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionUNIFEOB
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationUNESP, FCAV, Dept Vet Pathol, Lab Immunoparasitol, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationUNIFEOB, Sao Joao da Boa Vista, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Med Vet & Zootec, São Paulo, Brazil
dc.description.affiliationUnespUNESP, FCAV, Dept Vet Pathol, Lab Immunoparasitol, BR-14884900 Jaboticabal, SP, Brazil
dc.identifier.wosWOS:000249398200005
dc.rights.accessRightsAcesso aberto
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
dc.identifier.fileWOS000249398200005.pdf
dc.relation.isnodouble34581*
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