In vitro evaluation of three different biomaterials as scaffolds for canine mesenchymal stem cells
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Data
2013-05-01
Autores
Pereira-Junior, Oduvaldo Câmara Marques
Rahal, Sheila Canevese [UNESP]
Lima-Neto, João Ferreira
Landim-Alvarenga, Fernanda da Cruz [UNESP]
Monteiro, Frederico Ozanan Barros
Título da Revista
ISSN da Revista
Título de Volume
Editor
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
Resumo
PURPOSE: To evaluate in vitro ability the of three different biomaterials - purified hydroxyapatite, demineralized bone matrix and castor oil-based polyurethane - as biocompatible 3D scaffolds for canine bone marrow mesenchymal stem cell (MSC) intending bone tissue engineering. METHODS: MSCs were isolated from canine bone marrow, characterized and cultivated for seven days with the biomaterials. Cell proliferation and adhesion to the biomaterial surface were evaluated by scanning electron microscopy while differentiation into osteogenic lineage was evaluated by Alizarin Red staining and Sp7/Osterix surface antibody marker. RESULTS: The biomaterials allowed cellular growth, attachment and proliferation. Osteogenic differentiation occurred in the presence of hydroxyapatite, and matrix deposition commenced in the presence of the castor oil-based polyurethane. CONCLUSION: All the tested biomaterials may be used as mesenchymal stem cell scaffolds in cell-based orthopedic reconstructive therapy.
Descrição
Palavras-chave
Hydroxyapatites, Bone Matrix, Castor Oil, Polyurethanes, Stem Cells
Como citar
Acta Cirúrgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 28, n. 5, p. 353-360, 2013.