The use of scaning electron microscopy in postvaccinal evaluation of tracheal epithelium of Coturnix coturnix japonica
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Data
2003-02-01
Autores
Santin, Elizabeth [UNESP]
Lima, Fabiana S. [UNESP]
Paulillo, Antônio C. [UNESP]
Nakaghi, Laura S. O. [UNESP]
Maiorka, Alex [UNESP]
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Editor
Universidade Federal de Santa Maria (UFSM)
Resumo
Este experimento foi realizado para avaliar o emprego da microscopia eletrônica de varredura no estudo da reação respiratória pós-vacinal em epitélio traqueal de codornas (Coturnix coturnix japonica) imunizadas contra a doença de Newcastle. Foram utilizadas 36 codornas que foram distribuídas em quatro grupos, sendo: T1 ¾ grupo de aves controle (não vacinado), T2 ¾ grupo de aves vacinadas com a estirpe Ulster 2C, T3 ¾ grupo vacinado com a estirpe B1, T4 ¾ grupo de aves vacinadas com a estirpe LaSota. Independente do grupo experimental, as aves não apresentaram sinais clínicos detectáveis de reação respiratória pós-vacinal. Entretanto, na análise de fragmentos traqueais, ao microscópio eletrônico de varredura, observou-se que as codornas vacinadas com as estirpes B1 e LaSota desenvolveram descamação epitelial da traquéia, enquanto as aves vacinadas com a estirpe Ulster 2C não desenvolveram tal alteração, mostrando um epitélio traqueal íntegro, semelhante ao grupo controle.
This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 ¾ control birds (non-vaccinated); T2 ¾ birds vaccinated with Ulster 2C strain; t3 ¾ birds vaccinated with B1 strain; t4 ¾ birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by scanning electron microscopy showed that birds vaccinated with B1 and LaSota strains developed epithelial sloughing of the trachea, whereas those vaccinated Ulster 2C strain did not develop this change, demonstrating intact tracheal epithelium, similar to the control group.
This study aimed at evaluating the use of scanning electron microscopy in the study of the post-vaccinal respiratory reaction of the tracheal epithelium of quails (Coturnix coturnix japonica) immunized against Newcastle disease. A number of 36 quails were distributed into four groups: T1 ¾ control birds (non-vaccinated); T2 ¾ birds vaccinated with Ulster 2C strain; t3 ¾ birds vaccinated with B1 strain; t4 ¾ birds vaccinated with LaSota strain. Regardless the experimental group, birds did not show detectable clinical signs of post-vaccinal respiratory reaction. However, the analysis of tracheal fragments by scanning electron microscopy showed that birds vaccinated with B1 and LaSota strains developed epithelial sloughing of the trachea, whereas those vaccinated Ulster 2C strain did not develop this change, demonstrating intact tracheal epithelium, similar to the control group.
Descrição
Palavras-chave
Coturnix coturnix japonica, Newcastle disease, Scanning electron microscopy, respiratory post-vaccinal reaction, Coturnix coturnix japonica, Newcastle, Doença de, Microscopia eletronica de varredura, Reação respiratória pós-vacinal
Como citar
Ciência Rural. Universidade Federal de Santa Maria (UFSM), v. 33, n. 1, p. 121-124, 2003.