Comparação do efeito do ácido etilenodiaminotetracético (EDTA) e da heparina sobre os eritrócitos de avestruzes (Struthio camelus L.)
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Data
2010-06-01
Autores
Sabino, Antonio José
Trevelin, Silvia Cellone
Ciarlini, Paulo Cesar [UNESP]
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Universidade Federal de Viçosa (UFV)
Resumo
Anticoagulantes com diferentes formas de ação têm sido utilizados na hematologia aviária. Entretanto, poucos estudos foram publicados sobre seu efeito e sobre os parâmetros hematológicos dos avestruzes. Visando a preencher essa lacuna, realizou-se um estudo em que foi comparado o efeito de dois anticoagulantes de rotina (EDTA - 2 mg/mL sangue e da Heparina -10U/mL sangue) sobre os eritrócitos de avestruzes. Para tanto, foram avaliados a integridade, o volume globular e a morfometria dos eritrócitos de amostras sanguíneas de 20 aves, tratadas com ambos os anticoagulantes. A integridade eritrocitária foi estimada pelo grau de hemoglobina livre no plasma, sendo o volume globular obtido pelo método de micro-hematócrito e os dados morfométricos (área, diâmetro maior e menor) calculados automaticamente em sistema computadorizado de imagem digital de alta resolução. A hemólise estimada pela dosagem de hemoglobina plasmática, o volume globular, a área e o diâmetro máximo dos eritrócitos foram significativamente maiores nas amostras tratadas com EDTA. Para melhor preservar a integridade e a morfometria dos eritrócitos, concluiuse que a heparina é um anticoagulante mais adequado que o EDTA para avaliar as discrasias eritrocitárias dos avestruzes.
Anticoagulants with different modes of action have been used in avian hematology, however, few studies on their effects on the hematological parameters of ostriches were published. Seeking to fill this gap, the effect of two routine anticoagulants (EDTA - 2 mg/mL blood and Heparin - 10 U/mL blood) on erythrocytes of ostriches was compared. Blood samples of 20 ostriches, treated with both anticoagulants were evaluated for integrity, globular volume and morphometry of the erythrocytes. The erythrocyte integrity was estimated by the concentration of free hemoglobin in plasma. The globular volume was obtained by the method of microhematocrit and morphometric data (area, bigger and smaller diameters) were automatically calculated by a computerized system for high resolution digital image. Hemolysis estimated by plasma hemoglobin, packed cell volume, area and maximum diameter of erythrocytes were significantly higher in the samples treated with EDTA. To better preserve the integrity and morphometry of erythrocytes, it was concluded that the heparin rather than EDTA is a more appropriated anticoagulant to evaluate erythrocyte dyscrasia in ostriches.
Anticoagulants with different modes of action have been used in avian hematology, however, few studies on their effects on the hematological parameters of ostriches were published. Seeking to fill this gap, the effect of two routine anticoagulants (EDTA - 2 mg/mL blood and Heparin - 10 U/mL blood) on erythrocytes of ostriches was compared. Blood samples of 20 ostriches, treated with both anticoagulants were evaluated for integrity, globular volume and morphometry of the erythrocytes. The erythrocyte integrity was estimated by the concentration of free hemoglobin in plasma. The globular volume was obtained by the method of microhematocrit and morphometric data (area, bigger and smaller diameters) were automatically calculated by a computerized system for high resolution digital image. Hemolysis estimated by plasma hemoglobin, packed cell volume, area and maximum diameter of erythrocytes were significantly higher in the samples treated with EDTA. To better preserve the integrity and morphometry of erythrocytes, it was concluded that the heparin rather than EDTA is a more appropriated anticoagulant to evaluate erythrocyte dyscrasia in ostriches.
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Palavras-chave
Hemácias, interferência de anticoagulante, morfometria eritrocitária, técnica de colheita, hematologia aviária, Anticoagulant interference, avian hematology, blood collection techniques, erythrocyte morphometry, red blood cells (erythrocytes)
Como citar
Revista Ceres. Universidade Federal de Viçosa (UFV), v. 57, n. 3, p. 338-342, 2010.