Adsorptive potentiometric stripping analysis of nucleic acids at mercury electrodes
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Data
1997-08-29
Autores
Wang, J.
Grant, D. H.
Ozsoz, M.
Cai, X. H.
Tian, B. M.
Fernandes, JR
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
The application of adsorptive stripping potentiometry to the reductive detection of nucleic acids at mercury electrodes is reported. Compared to analogous voltammetric stripping modes, constant current potentiometric stripping analysis (PSA) effectively addresses the hydrogen discharge background problem, and hence greatly improves the characteristics of the superimposed cytosine/adenine (CA) reduction peak. Compared to earlier schemes for trace measurements of nucleic acids at mercury or carbon electrodes that rely on anodic signals arising from the guanine residue, convenient quantitation can now be carried out in connection with the cytosine and adenine residues. Variables influencing the adsorptive PSA response are explored and optimized. With five minute accumulation, the detection limits for tRNA, ssDNA and dsDNA are 30 mu g l(-1), 60 mu g l(-1) and 2 mg l(-1), respectively. Such different values reflect the strong dependence of the PSA CA signal upon the nucleic-acid structure. This allows the quantitation of ssDNA or tRNA in the presence of dsDNA, and offers new possibilities for electrochemical studies of DNA structure and interactions.
Descrição
Palavras-chave
DNA, RNA, mercury electrode, potentiometric stripping analysis, adsorptive stripping voltammetry
Como citar
Analytica Chimica Acta. Amsterdam: Elsevier B.V., v. 349, n. 1-3, p. 77-83, 1997.