Assay of Serum Glutamic-Oxaloacetic Transaminase Using Malate Dehydrogenase Preparation Obtained from Streptomyces aureofaciens
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Data
1995-01-01
Autores
Yamanaka, H.
Laluce, C.
Deoliveiraneto, G.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Marcel Dekker Inc
Resumo
A modified spectrophotometric method for serum glutamic-oxaloacetic transaminase (SGOT) assay was developed. A crude cell-free extract from Streptomyces aureofaciens which showed a high level of malate dehydrogenase (MDH) activity (E.C. 1.1.1.37) was used as the enzymatic indicator. The lyophilized microbial preparation was used without previous purification and was quite stable under refrigeration for one year. Serum sample assays using both the method utilizing the crude cell extract and an enzymatic commercial kit showed good correlation.
Descrição
Palavras-chave
TRANSAMINASE, SGOT, MALATE DEHYDROGENASE, STREPTOMYCES
Como citar
Analytical Letters. New York: Marcel Dekker Inc., v. 28, n. 13, p. 2305-2316, 1995.