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dc.contributor.authorRibeiro, D. A.
dc.contributor.authorSalvadori, Daisy Maria Favero [UNESP]
dc.contributor.authorMarques, MEA
dc.date.accessioned2014-05-20T15:24:29Z
dc.date.available2014-05-20T15:24:29Z
dc.date.issued2005-12-01
dc.identifierhttp://dx.doi.org/10.1111/j.0959-9673.2005.00444.x
dc.identifier.citationInternational Journal of Experimental Pathology. Oxford: Blackwell Publishing, v. 86, n. 6, p. 375-381, 2005.
dc.identifier.issn0959-9673
dc.identifier.urihttp://hdl.handle.net/11449/35087
dc.description.abstract4-Nitroquinoline 1-oxide (4NQO)-induced rat tongue carcinogenesis is a useful model for studying oral squamous cell carcinoma. The aim of this study was to investigate the expression of bcl-2 and bax during tongue carcinogenesis induced by 4NQO. Male Wistar rats were distributed into three groups of 10 animals each and treated with 50 ppm 4NQO solution through their drinking water for 4, 12 or 20 weeks. Ten animals were used as negative control. Although no histological changes were induced in the epithelium after 4 weeks of carcinogen exposure, bcl-2 and bax were over-expressed (P < 0.01) in all layers of the 'normal' epithelium. The expression levels were the same in all layers of epithelium for both the antibodies used (bcl-2 or bax). In dysplastic lesions at 12 weeks following carcinogen administration, the levels of bcl-2 and bax expression did not increase when compared to negative control with the immunoreactivity for bcl-2 being restricted to the superficial layer of epithelium. In well-differentiated squamous cell carcinoma induced after 20 weeks of treatment with 4NQO, bcl-2 was expressed in some cells of tumour islands. on the other hand, immunostaining for bax was widely observed at the tumour nests. The labelling index for bcl-2 and bax showed an increase (P < 0.05) after only 4 weeks of 4NQO administration. In conclusion, our results suggest that abnormalities in the apoptosis pathways are associated with the development of persistent clones of mutated-epithelial cells in the oral mucosa. Bcl-2 and bax expression appears to be associated with a risk factor in the progression of oral cancer.en
dc.format.extent375-381
dc.language.isoeng
dc.publisherBlackwell Publishing
dc.relation.ispartofInternational Journal of Experimental Pathology
dc.sourceWeb of Science
dc.subject4-nitroquinoline 1-oxidept
dc.subjectbaxpt
dc.subjectbcl-2pt
dc.subjectoral cancerpt
dc.titleAbnormal expression of bcl-2 and bax in rat tongue mucosa during the development of squamous cell carcinoma induced by 4-nitroquinoline 1-oxideen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderBlackwell Publishing
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Estadual Paulista Julio Mesquita Filho, Botucatu Med Sch, Fac Med Botucatu, TOXICAN,Ctr Genotoxins & Carcinogens Evaluat,Dept, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Julio Mesquita Filho, Botucatu Med Sch, Fac Med Botucatu, TOXICAN,Ctr Genotoxins & Carcinogens Evaluat,Dept, BR-18618000 Botucatu, SP, Brazil
dc.identifier.doi10.1111/j.0959-9673.2005.00444.x
dc.identifier.wosWOS:000233517500003
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Medicina, Botucatupt
dc.identifier.lattes5051118752980903
unesp.author.lattes5051118752980903
unesp.author.orcid0000-0001-9323-3134[2]
dc.relation.ispartofjcr1.938
dc.relation.ispartofsjr0,712
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