Automated system of seedling image analysis (SVIS) and electrical conductivity to assess sun hemp seed vigor
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Data
2012-01-01
Autores
Silva, Clíssia Barboza da [UNESP]
Lopes, Magnólia de Mendonça [UNESP]
Marcos-Filho, Julio
Vieira, Roberval Daiton [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Associação Brasileira de Tecnologia de Sementes (ABRATES)
Resumo
O desenvolvimento de novos procedimentos para rápida obtenção de informações precisas sobre o potencial fisiológico é essencial para o desenvolvimento de programas de controle de qualidade pelas empresas produtoras de sementes. Neste estudo, a eficácia de um sistema automatizado de análise de imagens de plântulas (Seed Vigor Imaging System - SVIS) na determinação do potencial fisiológico de sementes de crotalária, bem como sua associação com o teste de condutividade elétrica foram avaliados. As avaliações pelo SVIS foram feitas aos três e quatro dias após a semeadura e dados referentes a índice de vigor, comprimento e uniformidade do crescimento das plântulas foram coletados. O teste de condutividade elétrica foi realizado em repetições de 50 sementes colocadas em recipientes contendo 75 mL de água desionizada, a 25 ºC e as leituras, realizadas após 1, 2, 4, 8 e 16 horas de embebição. As medidas de condutividade elétrica com 4 ou 8 horas e o uso do SVIS em plântulas com três dias de idade podem detectar efetivamente diferenças no vigor de diferentes lotes de sementes de crotalária.
The development of new procedures for quickly obtaining accurate information on the physiological potential of seed lots is essential for developing quality control programs for the seed industry. In this study, the effectiveness of an automated system of seedling image analysis (Seed Vigor Imaging System - SVIS) in determining the physiological potential of sun hemp seeds and its relationship with electrical conductivity tests, were evaluated. SVIS evaluations were performed three and four days after sowing and data on the vigor index and the length and uniformity of seedling growth were collected. The electrical conductivity test was made on 50 seed replicates placed in containers with 75 mL of deionised water at 25 ºC and readings were taken after 1, 2, 4, 8 and 16 hours of imbibition. Electrical conductivity measurements at 4 or 8 hours and the use of the SVIS on 3-day old seedlings can effectively detect differences in vigor between different sun hemp seed lots.
The development of new procedures for quickly obtaining accurate information on the physiological potential of seed lots is essential for developing quality control programs for the seed industry. In this study, the effectiveness of an automated system of seedling image analysis (Seed Vigor Imaging System - SVIS) in determining the physiological potential of sun hemp seeds and its relationship with electrical conductivity tests, were evaluated. SVIS evaluations were performed three and four days after sowing and data on the vigor index and the length and uniformity of seedling growth were collected. The electrical conductivity test was made on 50 seed replicates placed in containers with 75 mL of deionised water at 25 ºC and readings were taken after 1, 2, 4, 8 and 16 hours of imbibition. Electrical conductivity measurements at 4 or 8 hours and the use of the SVIS on 3-day old seedlings can effectively detect differences in vigor between different sun hemp seed lots.
Descrição
Palavras-chave
Crotalaria juncea, seedling image, Software, Crotalária juncea, imagem de plântulas, Software
Como citar
Revista Brasileira de Sementes. Associação Brasileira de Tecnologia de Sementes, v. 34, n. 1, p. 55-60, 2012.