Solubilização do fosfato de ferro em meio de cultura
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Data
2008-04-01
Autores
Barroso, Cinthya Babá
Nahas, Ely [UNESP]
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ISSN da Revista
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Editor
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
Resumo
O objetivo deste trabalho foi avaliar a eficiência de solubilização de FePO4 por Aspergillus niger, em meio de cultura com diferentes fontes de carbono (C) e nitrogênio (N) e com concentrações crescentes de fosfato. A quantidade de fosfato solúvel, acidez e pH final foram determinados após o crescimento do fungo, em cultura estacionária a 30ºC. A eficiência de solubilização aumentou conforme o crescimento do fungo, atingiu o máximo no 11º dia (68%) e depois regrediu. Das fontes de C e de N testadas, as maiores eficiências de solubilização foram obtidas com manitol (21%) e ácido glutâmico (17%). Com o aumento da concentração de fosfato (0 a 1.330 µg mL-1), a máxima eficiência de solubilização (70%) foi obtida com 330 µg mL-1 de PO4(3-) e decresceu até 47% com 1.330 µg mL-1. A produção de ácidos foi o principal mecanismo de solubilização do FePO4, com base na correlação positiva e significativa entre a produção de fosfato e a acidez.
The objective of this work was to evaluate the efficiency of solubilization of FePO4 by Aspergillus niger, in culture medium with different carbon (C) and nitrogen (N) sources, and enhancing concentrations of phosphate. The amount of soluble phosphate, acidity and final pH were determined after the fungus growth in a stationary culture at 30ºC. Efficiency of solubilization increased according to fungus growth, reaching the maximum at the 11th day (68%) and later decreased. In relation to C and N sources, the largest efficiencies of solubilization were found with mannitol (21%) and glutamic acid (17%). Increasing phosphate concentration (0 to 1,330 µg mL-1), maximal efficiency of solubilization (70%) was found with 330 µg mL-1 PO4(3-), and decreased up to 47% with 1,330 µg mL-1 PO4(3-). The amount of acids produced was the factor that more influenced FePO4 solubilization, based in the positive and significant correlation between phosphate and acidity production.
The objective of this work was to evaluate the efficiency of solubilization of FePO4 by Aspergillus niger, in culture medium with different carbon (C) and nitrogen (N) sources, and enhancing concentrations of phosphate. The amount of soluble phosphate, acidity and final pH were determined after the fungus growth in a stationary culture at 30ºC. Efficiency of solubilization increased according to fungus growth, reaching the maximum at the 11th day (68%) and later decreased. In relation to C and N sources, the largest efficiencies of solubilization were found with mannitol (21%) and glutamic acid (17%). Increasing phosphate concentration (0 to 1,330 µg mL-1), maximal efficiency of solubilization (70%) was found with 330 µg mL-1 PO4(3-), and decreased up to 47% with 1,330 µg mL-1 PO4(3-). The amount of acids produced was the factor that more influenced FePO4 solubilization, based in the positive and significant correlation between phosphate and acidity production.
Descrição
Palavras-chave
Aspergillus niger, titratable acidity, Carbon source, nitrogen sources, soluble phosphate, Aspergillus niger, Acidez titulável, Fontes de carbono, fontes de nitrogênio, fosfato solúvel
Como citar
Pesquisa Agropecuária Brasileira. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Informação TecnológicaPesquisa Agropecuária Brasileira, v. 43, n. 4, p. 529-535, 2008.