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dc.contributor.authorAnjos, A. R.
dc.contributor.authorCalvi, S. A.
dc.contributor.authorFerracini, R.
dc.contributor.authorPeracoli, MTS
dc.contributor.authorSilva, C. L.
dc.contributor.authorSoares, AMVC
dc.identifier.citationMedical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 40, n. 4, p. 377-382, 2002.
dc.description.abstractThe polysaccharide fraction of Paracoccidioides brasiliensis mycelial cell wall (F1 fraction), the active component of which is composed of beta-glucan, was investigated in regard to the activation of human monocytes for fungal killing. The cells were primed with interferon-gamma (IFN-gamma) or F1 (100 and 200 mug ml(-1)) or F1 (100 and 200 mug ml(-1)) plus IFN-gamma for 24 h and then evaluated for H2O2 release. In other experiments, the cells were pretreated with the same stimuli, challenged with a virulent strain of P. brasiliensis and evaluated for fungicidal activity and levels of tumor necrosis factor (TNF-alpha) in the supernatants. F1 increased the levels of H2O2 in a similar manner to IFN-gamma. However, a synergistic effect between these two activators was not detected. on the contrary, a significant fungicidal activity was only obtained after priming with IFN-gamma plus F1. This higher activity was associated with high levels of TNF-alpha in the supernatants of the cocultures. Overall, P. brasiliensis F1 fraction induced human monocytes to release relatively high levels of TNF-alpha, which, in combination with IFN-gamma, is responsible for the activation of human monocytes for effective killing of P. brasiliensis.en
dc.publisherB I O S Scientific Publishers Ltd
dc.relation.ispartofMedical Mycology
dc.sourceWeb of Science
dc.subjectfungicidal activitypt
dc.subjectParacoccidioides brasiliensispt
dc.titleRole of Paracoccidioides brasiliensis cell wall fraction containing beta-glucan in tumor necrosis factor-alpha production by human monocytes: correlation with fungicidal activityen
dcterms.rightsHolderB I O S Scientific Publishers Ltd
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.description.affiliationSão Paulo State Univ, Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.description.affiliationUniv São Paulo, Sch Med, Dept Parasitol Microbiol & Immunol, BR-05508 São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Biosci Inst, Dept Microbiol & Immunol, Botucatu, SP, Brazil
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt[4][5]
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