Colorimetric assay of ethanol using alcohol dehydrogenase from dry baker's yeast
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Data
2007-02-05
Autores
Zanon, Juliana P.
Peres, Maristela F. S.
Gattas, Edwil Aparecida de Lucca [UNESP]
Título da Revista
ISSN da Revista
Título de Volume
Editor
Elsevier B.V.
Resumo
Alcohol dehydrogenases (ADHs) are oxidoreductases present in animal tissues, plants, and microorganisms. These enzymes attract major scientific interest for the evolutionary perspectives, afforded by their wide occurrence in nature, and for their use in synthesis, thanks to their broad substrate specificity and stereoselectivity. In the present study, the standardization of the activity of the alcohol dehydrogenase from baker's yeast was accomplished, and the pH and temperature stability showed, that the enzyme presented a high stability to pH 6.0-7.0 and the thermal stability were completely maintained up to 50 degrees C during 1 h. The assays of ethanol (detection range 1-5 mM or 4.6 x 10(-2) to 23.0 x 10(-2) g/L) in different samples in alcoholic beverages, presented a maximum deviation of only 7.2%. The standard curve and the analytic curve of this method meet the conditions of precision, sensitivity, simplicity, and low cost, required for a useable analytical method. (c) 2006 Elsevier B.V. All rights reserved.
Descrição
Palavras-chave
alcohol dehydrogenase, colorimetric assay of ethanol, baker's yeast
Como citar
Enzyme and Microbial Technology. New York: Elsevier B.V., v. 40, n. 3, p. 466-470, 2007.