Influence of free amino acids on clavulanic acid production by Streptomyces clavuligerus in synthetic medium
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Data
2006-02-01
Autores
Trovatti, Eliane
Badino, Alberto Colli
Hokka, Carlos Osamu
Gonsales da Costa Araujo, Maria Lucia
Título da Revista
ISSN da Revista
Título de Volume
Editor
Inst Tecnologia Parana
Resumo
The effect of nitrogen source on clavulanic acid production was investigated in shake flasks. Media containing asparagine plus one of several different amino acids or a combination of two amino acids was tested. The best result, ca. 180 mg/L clavulanic acid, CA, in 60 h, was obtained with the lysine-tyrosine pair In an aerated and agitated fermentor this medium led to CA concentrations of ca. 210 mg/L, a remarkable production for synthetic medium utilization. Amino acids analysis during cultivation indicated that, while asparagine was consumed rapidly, lysine and tyrosine were metabolized slowly, promoting CA production.
Descrição
Palavras-chave
clavulanic acid, b-lactamase inhibitor, S. clavuligerus, synthetic medium, amino acids
Como citar
Brazilian Archives of Biology and Technology. Curitiba-Paraná: Inst Tecnologia Parana, v. 49, p. 173-179, 2006.