Thermal Characterization and Cytotoxicity of Complexes Formed by Papain and Cyclodextrin
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Data
2007-12-01
Autores
Varca, Gustavo H. C.
Andreo-Filho, Newton
Fraceto, Leonardo F. [UNESP]
Kaneko, Telma M.
Ferraz, Humberto G.
Esteves, Natalia M.
Issa, Michelle G.
Mathor, Monica B.
Lopes, Patricia S.
Título da Revista
ISSN da Revista
Título de Volume
Editor
Springer
Resumo
Papain is a proteolytic enzyme with restricted applications due to its limited stability. Cyclodextrins are widely used in pharmaceutical formulations once they are able to form complexes with other molecules, improving their stability and bioavailability. The purpose of the present paper was to analyze complexes formed by papain/hydroxypropyl-beta-cyclodextrin and papain/beta-cyclodextrin by thermal analysis and cytotoxicity tests to verify their possible interactions and toxicological behavior. Complex solutions were prepared at different molar ratios and collected as a function of time to be lyophilized and analyzed. Results showed changes in endothermic events and cytotoxicity profiles. A complex formation for both complexes is observed; nevertheless, beta-cyclodextrin was able to change the enzyme characteristics more efficiently.
Descrição
Palavras-chave
Papain, Cyclodextrin, Cytotoxicity, Differential scanning calorimetry, Fibroblasts, Human keratinocytes
Como citar
Journal of Biological Physics. Dordrecht: Springer, v. 33, n. 5-6, p. 463-475, 2007.