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dc.contributor.authorMurakami, M. T.
dc.contributor.authorMichelan-Duarte, S.
dc.contributor.authorCintra, ACO
dc.contributor.authorArni, R. K.
dc.date.accessioned2014-05-20T15:29:06Z
dc.date.available2014-05-20T15:29:06Z
dc.date.issued2004-12-01
dc.identifierhttp://dx.doi.org/10.1016/j.bbapap.2004.08.008
dc.identifier.citationBiochimica Et Biophysica Acta-proteins and Proteomics. Amsterdam: Elsevier B.V., v. 1703, n. 1, p. 79-81, 2004.
dc.identifier.issn1570-9639
dc.identifier.urihttp://hdl.handle.net/11449/38760
dc.description.abstractSnake venom PLA(2)s have been extensively studied due to their role in mediating and disrupting physiological processes such as coagulation, platelet aggregation and myotoxicity. The Ca2+ ion bound to the putative calcium-binding loop is essential for hydrolytic activity. We report the crystallization in the presence and absence of Ca2+ and X-ray diffraction data collection at 1.60 Angstrom (with Ca2+) and 1.36 Angstrom (without Ca2+) of an Asp49 PLA(2) from Bothrops jararacussu venom. The crystals belong to orthorhombic space group C222(1). Initial refinement and electron density analysis indicate significant conformational. changes upon Ca2+ binding. (C) 2004 Elsevier B.V. All fights reserved.en
dc.format.extent79-81
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiochimica et Biophysica Acta: Proteins and Proteomics
dc.sourceWeb of Science
dc.subjectcalcium-binding looppt
dc.subjectAsp49 PLA(2)pt
dc.subjectcrystallizationpt
dc.subjectX-ray diffractionpt
dc.titleCrytallization and high-resolution X-ray diffraction data collection of an Asp49 PLA(2) from Bothrops jararacussu venom both in the presence and absence of Ca2+ ionsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv São Paulo, Dept Anal Clin Toxicol & Bromatol, Fac Ciências Farmaceut, BR-14049 Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Fis, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Fis, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.identifier.doi10.1016/j.bbapap.2004.08.008
dc.identifier.wosWOS:000225621800009
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências Letras e Ciências Exatas, São José do Rio Pretopt
dc.identifier.lattes9162508978945887
dc.identifier.orcid0000-0003-2460-1145
unesp.author.lattes9162508978945887[4]
unesp.author.orcid0000-0002-0405-8010[1]
unesp.author.orcid0000-0003-2460-1145[4]
dc.relation.ispartofjcr2.609
dc.relation.ispartofsjr1,170
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