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dc.contributor.authorCarmona, E. C.
dc.contributor.authorHan, S. W.
dc.contributor.authorPalma, Mario Sergio [UNESP]
dc.contributor.authorRossi, A.
dc.date.accessioned2014-05-20T15:29:26Z
dc.date.available2014-05-20T15:29:26Z
dc.date.issued1996-02-01
dc.identifierhttp://dx.doi.org/10.1016/0031-9422(95)00531-5
dc.identifier.citationPhytochemistry. Oxford: Pergamon-Elsevier B.V., v. 41, n. 2, p. 345-348, 1996.
dc.identifier.issn0031-9422
dc.identifier.urihttp://hdl.handle.net/11449/39029
dc.description.abstractA polynucleotide (or a fragment of RNA) was purified to apparent homogeneity by HPLC from mycelium of the wild strain 74A of the mould Neurospora crassa, after growth on sucrose and in the presence of saturating amounts of inorganic phosphate (Pi) for 72 hr at 30 degrees. The M(r) was ca 20000 as determined by HPLC at pH 6.8. Polynucleotide synthesis ranged from 4.0 to 6.5 mu g polynucleotide per mg dry mycelium in mycelium of the wild strain 74A and the various phosphorus regulatory and structural mutant strains of the mould N. crassa. Kinetic data showed that the polynucleotide interacts with mycelial Pi-repressible alkaline phosphatase by inhibiting its p-nitrophenylphosphatase activity and by protecting the enzyme against thermal inactivation in the presence of high concentrations of ammonium sulphate.en
dc.format.extent345-348
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPhytochemistry
dc.sourceWeb of Science
dc.subjectNeurospora crassapt
dc.subjectfungipt
dc.subjectpolynucleotidept
dc.subjectalkaline phosphatasept
dc.subjectRNA-binding proteinspt
dc.titleProperties of a polynucleotide synthesized by strain 74a of Neurospora crassaen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUSP,FFCLRP,DEPT QUIM,BR-14040901 RIBEIRAO PRET,SP,BRAZIL
dc.description.affiliationUNESP,IB,DEPT BIOQUIM & MICROBIOL,BR-13506900 RIO CLARO,SP,BRAZIL
dc.description.affiliationUNESP,IB,DEPT BIOL,BR-13506900 RIO CLARO,SP,BRAZIL
dc.description.affiliationUnespUNESP,IB,DEPT BIOQUIM & MICROBIOL,BR-13506900 RIO CLARO,SP,BRAZIL
dc.description.affiliationUnespUNESP,IB,DEPT BIOL,BR-13506900 RIO CLARO,SP,BRAZIL
dc.identifier.doi10.1016/0031-9422(95)00531-5
dc.identifier.wosWOS:A1996TT44800002
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt
dc.identifier.lattes4110421764783871
dc.identifier.lattes2901888624506535
unesp.author.lattes4110421764783871
unesp.author.lattes2901888624506535
unesp.author.orcid0000-0002-0230-5735[1]
unesp.author.orcid0000-0002-7363-8211[3]
dc.relation.ispartofjcr3.186
dc.relation.ispartofsjr1,048
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