Now showing items 1-10 of 29
ALLOSTERIC MODULATION BY ATP, CALCIUM AND MAGNESIUM-IONS OF RAT OSSEOUS PLATE ALKALINE-PHOSPHATASE
(Biochimica Et Biophysica Acta, 1993-09-03) [Artigo]
Alkaline phosphatase from rat osseous plate is allosterically modulated by ATP, calcium and magnesium at pH 7.5. At pH 9.4, the hydrolysis of ATP and PNPP follows Michaelis-Menten kinetics with K0.5 values of 154 muM and ...
OSSEOUS PLATE ALKALINE-PHOSPHATASE IS ANCHORED BY GPI
(Brazilian Journal of Medical and Biological Research, 1994-02-01) [Artigo]
Alkaline phosphatase activity was released up to 100% from the membrane by using 0.1 U of phosphatidylinositol-specific phospholipase C from B. thuringiensis. The Mr of solubilized enzyme was 145,000 by Sephacryl S-300 gel ...
Nutritionally induced changes in endosperm of shrunken-1 and brittle-2 maize kernels grown in vitro
(Crop Science, 1996-07-01) [Artigo]
Although mineral nutrition affects maize (Zea mays L.) yield by controlling starch deposition in kernels, the mechanisms involved are largely unknown. Our objectives were to examine this relationship by nutritionally and ...
EFFECT OF THE QUANTITY OF DIETARY AMINO-ACIDS ON EGG-PRODUCTION AND LAYINGS BY CERATITIS-CAPITATA (DIPTERA, TEPHRITIDAE)
(Brazilian Journal of Medical and Biological Research, 1990-01-01) [Artigo]
Efeito da somatotropina bovina recombinante no desempenho e nas características químicas da carne de bovinos
(Pesquisa Agropecuária Brasileira, 1998-02-01) [Artigo]
The objective of this study was to evaluate the effects of the use of recombinant bovine somatotropin (rbST) in weight gain, feeding efficiency and chemical characteristics of meat of young bulls. Fifty nine young bulls ...
Heat treatment and inactivation of trypsin-chymotrypsin inhibitors and lectins from beans (Phaseolus vulgaris L.)
(Journal of Food Biochemistry, 1997-08-01) [Artigo]
This work investigates some factors affecting the inactivation of common bean trypsin inhibitor and phytohemagglutin. Trypsin inhibitor activity was totally stable to heat treatment (30 min, 97C) in the total protein ...
Effect of calcium ions on rat osseous plate alkaline phosphatase activity
(Journal of Inorganic Biochemistry, 1997-11-01) [Artigo]
Rat osseous plate alkaline phosphatase is a metalloenzyme with two binding sites for Zn2+ (sites I and III) and one for Mg2+ (site II). This enzyme is stimulated synergistically by Zn2+ and Mg2+ (Ciancaglini et al., 1992) ...
Kinetic characterization of a membrane-specific ATPase from rat osseous plate and its possible significance on endochondral ossification
(Biochimica et Biophysica Acta: Biomembranes, 1998-01-05) [Artigo]
Treatment with phosphatidylinositol-specific phospholipase C of rat osseous plate membranes released up to 90-95% of alkaline phosphatase, but a specific ATPase activity (optimum pH = 7.5) remained bound to the membrane. ...
FUNCTIONAL ALPHA-TROPOMYOSIN PRODUCED IN ESCHERICHIA-COLI - A DIPEPTIDE EXTENSION CAN SUBSTITUTE THE AMINO-TERMINAL ACETYL GROUP
(Journal of Biological Chemistry, 1994-04-08) [Artigo]
Unlike the muscle protein, alpha-tropomyosin expressed in Escherichia coli does not bind actin, does not exhibit head-to-tail polymerization, and does not inhibit actomyosin ATPase activity in the absence of troponin. The ...
CONCERTED ACTION OF THE HIGH-AFFINITY CALCIUM-BINDING SITES IN SKELETAL-MUSCLE TROPONIN-C
(Journal of Biological Chemistry, 1995-04-28) [Artigo]
Mutants of each of the four divalent cation binding sites of chicken skeletal muscle troponin C (TnC) were constructed using site directed mutagenesis to convert Asp to Ala at the first coordinating position in each site. ...