Now showing items 1-4 of 4
Inorganic pyrophosphate-phosphohydrolytic activity associated with rat osseous plate alkaline phosphatase
(Cellular and Molecular Biology, 1998-03-01) [Artigo]
Purified membrane-bound alkaline phosphatase from rat osseous plate hydrolyzed pyrophosphate in the presence of magnesium ions, with a specific activity of 92.7 U/mg. Optimal apparent pH for pyrophosphatase activity was ...
Kinetic characterization of a membrane-specific ATPase from rat osseous plate and its possible significance on endochondral ossification
(Biochimica et Biophysica Acta: Biomembranes, 1998-01-05) [Artigo]
Treatment with phosphatidylinositol-specific phospholipase C of rat osseous plate membranes released up to 90-95% of alkaline phosphatase, but a specific ATPase activity (optimum pH = 7.5) remained bound to the membrane. ...
Short communication: Resazurin reducing time as an indicator of Bradyrhizobium viable cell count
(World Journal of Microbiology and Biotechnology, 1998-01-01) [Artigo]
A method based on the use of resazurin (RSZ) is described to determine the number of viable Bradyrhizobium japonicum cells in culture medium. The observation of RSZ reduction can be done spectrophotometrically or visually. ...
Allosteric modulation of pyrophosphatase activity of rat osseous plate alkaline phosphatase by magnesium ions
(International Journal of Biochemistry & Cell Biology, 1998-01-01) [Artigo]
Pyrophosphatase activity of rat osseous plate alkaline phosphatase was studied at different concentrations of calcium and magnesium ions. with the aim of characterizing the modulation of enzyme activity by these metals. ...