Now showing items 1-5 of 5
Characterization of the phosphatidylinositol-specific phospholipase C-released form of rat osseous plate alkaline phosphatase and its possible significance on endochondral ossification
(Molecular And Cellular Biochemistry, 1995-11-22) [Artigo]
Alkaline phosphatase activity was released up to 100% from the membrane by incubating the rat osseous plate membrane-bound enzyme with phosphatidylinositol-specific phospholipase C. The molecular weight of the released ...
Inorganic pyrophosphate-phosphohydrolytic activity associated with rat osseous plate alkaline phosphatase
(Cellular and Molecular Biology, 1998-03-01) [Artigo]
Purified membrane-bound alkaline phosphatase from rat osseous plate hydrolyzed pyrophosphate in the presence of magnesium ions, with a specific activity of 92.7 U/mg. Optimal apparent pH for pyrophosphatase activity was ...
Kinetic characterization of a membrane-specific ATPase from rat osseous plate and its possible significance on endochondral ossification
(Biochimica et Biophysica Acta: Biomembranes, 1998-01-05) [Artigo]
Treatment with phosphatidylinositol-specific phospholipase C of rat osseous plate membranes released up to 90-95% of alkaline phosphatase, but a specific ATPase activity (optimum pH = 7.5) remained bound to the membrane. ...
Allosteric modulation of pyrophosphatase activity of rat osseous plate alkaline phosphatase by magnesium ions
(International Journal of Biochemistry & Cell Biology, 1998-01-01) [Artigo]
Pyrophosphatase activity of rat osseous plate alkaline phosphatase was studied at different concentrations of calcium and magnesium ions. with the aim of characterizing the modulation of enzyme activity by these metals. ...
OSSEOUS PLATE ALKALINE-PHOSPHATASE IS ANCHORED BY GPI
(Brazilian Journal of Medical and Biological Research, 1994-02-01) [Artigo]
Alkaline phosphatase activity was released up to 100% from the membrane by using 0.1 U of phosphatidylinositol-specific phospholipase C from B. thuringiensis. The Mr of solubilized enzyme was 145,000 by Sephacryl S-300 gel ...