Now showing items 1-10 of 21
Characterization of the phosphatidylinositol-specific phospholipase C-released form of rat osseous plate alkaline phosphatase and its possible significance on endochondral ossification
(Molecular And Cellular Biochemistry, 1995-11-22) [Artigo]
Alkaline phosphatase activity was released up to 100% from the membrane by incubating the rat osseous plate membrane-bound enzyme with phosphatidylinositol-specific phospholipase C. The molecular weight of the released ...
Nutritionally induced changes in endosperm of shrunken-1 and brittle-2 maize kernels grown in vitro
(Crop Science, 1996-07-01) [Artigo]
Although mineral nutrition affects maize (Zea mays L.) yield by controlling starch deposition in kernels, the mechanisms involved are largely unknown. Our objectives were to examine this relationship by nutritionally and ...
EFFECT OF THE QUANTITY OF DIETARY AMINO-ACIDS ON EGG-PRODUCTION AND LAYINGS BY CERATITIS-CAPITATA (DIPTERA, TEPHRITIDAE)
(Brazilian Journal of Medical and Biological Research, 1990-01-01) [Artigo]
Inorganic pyrophosphate-phosphohydrolytic activity associated with rat osseous plate alkaline phosphatase
(Cellular and Molecular Biology, 1998-03-01) [Artigo]
Purified membrane-bound alkaline phosphatase from rat osseous plate hydrolyzed pyrophosphate in the presence of magnesium ions, with a specific activity of 92.7 U/mg. Optimal apparent pH for pyrophosphatase activity was ...
Sucrose and nitrogen supplies regulate growth of maize kernels
(Annals of Botany, 1999-12-01) [Artigo]
The growth of maize (Zea mays L.) kernels depends on the availability of carbon (C) and nitrogen (N) assimilates supplied by the mother plant and the capacity of the kernel to use them. Our objectives were to study the ...
Heat treatment and inactivation of trypsin-chymotrypsin inhibitors and lectins from beans (Phaseolus vulgaris L.)
(Journal of Food Biochemistry, 1997-08-01) [Artigo]
This work investigates some factors affecting the inactivation of common bean trypsin inhibitor and phytohemagglutin. Trypsin inhibitor activity was totally stable to heat treatment (30 min, 97C) in the total protein ...
The Effects of N Helix Deletion and Mutant F29W on the Ca2+ Binding and Functional Properties of Chicken Skeletal Muscle Troponin C
(Journal of Biological Chemistry, 1994-05-27) [Artigo]
To assess the structural and functional significance of the N helix (residues 3-13) of avian recombinant troponin C (rTnC), we have constructed NHdel, in which residues 1-11 have been deleted, both in rTnC and in the ...
Effect of calcium ions on rat osseous plate alkaline phosphatase activity
(Journal of Inorganic Biochemistry, 1997-11-01) [Artigo]
Rat osseous plate alkaline phosphatase is a metalloenzyme with two binding sites for Zn2+ (sites I and III) and one for Mg2+ (site II). This enzyme is stimulated synergistically by Zn2+ and Mg2+ (Ciancaglini et al., 1992) ...
Kinetic characterization of a membrane-specific ATPase from rat osseous plate and its possible significance on endochondral ossification
(Biochimica et Biophysica Acta: Biomembranes, 1998-01-05) [Artigo]
Treatment with phosphatidylinositol-specific phospholipase C of rat osseous plate membranes released up to 90-95% of alkaline phosphatase, but a specific ATPase activity (optimum pH = 7.5) remained bound to the membrane. ...
CONCERTED ACTION OF THE HIGH-AFFINITY CALCIUM-BINDING SITES IN SKELETAL-MUSCLE TROPONIN-C
(Journal of Biological Chemistry, 1995-04-28) [Artigo]
Mutants of each of the four divalent cation binding sites of chicken skeletal muscle troponin C (TnC) were constructed using site directed mutagenesis to convert Asp to Ala at the first coordinating position in each site. ...