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dc.contributor.authorCaseli, Luciano
dc.contributor.authorMoraes, Marli L.
dc.contributor.authorZucolotto, Valtencir
dc.contributor.authorFerreira, Marystela
dc.contributor.authorNobre, Thatyane M.
dc.contributor.authorZaniquelli, Maria Elisabete D.
dc.contributor.authorRodrigues Filho, Ubirajara P.
dc.contributor.authorOliveira, Osvaldo N.
dc.date.accessioned2014-05-20T15:29:54Z
dc.date.available2014-05-20T15:29:54Z
dc.date.issued2006-09-26
dc.identifierhttp://dx.doi.org/10.1021/la061799g
dc.identifier.citationLangmuir. Washington: Amer Chemical Soc, v. 22, n. 20, p. 8501-8508, 2006.
dc.identifier.issn0743-7463
dc.identifier.urihttp://hdl.handle.net/11449/39378
dc.description.abstractThis paper reports the surface activity of phytase at the air-water interface, its interaction with lipid monolayers, and the construction of a new phytic acid biosensor on the basis of the Langmuir-Blodgett (LB) technique. Phytase was inserted in the subphase solution of dipalmitoylphosphatidylglycerol (DPPG) Langmuir monolayers, and its incorporation to the air-water interface was monitored with surface pressure measurements. Phytase was able to incorporate into DPPG monolayers even at high surface pressures, ca. 30 mN/m, under controlled ionic strength, pH, and temperature. Mixed Langmuir monolayers of phytase and DPPG were characterized by surface pressure-area and surface potential-area isotherms, and the presence of the enzyme provided an expansion in the monolayers ( when compared to the pure lipid at the interface). The enzyme incorporation also led to significant changes in the equilibrium surface compressibility (in-plane elasticity), especially in liquid-expanded and liquid-condensed regions. The dynamic surface elasticity for phytase-containing interfaces was investigated using harmonic oscillation and axisymmetric drop shape analysis. The insertion of the enzyme at DPPG monolayers caused an increase in the dynamic surface elasticity at 30 mN m(-1), indicating a strong interaction between the enzyme and lipid molecules at a high-surface packing. Langmuir-Blodgett (LB) films containing 35 layers of mixed phytase-DPPG were characterized by ultraviolet-visible and fluorescence spectroscopy and crystal quartz microbalance nanogravimetry. The ability in detecting phytic acid was studied with voltammetric measurements.en
dc.format.extent8501-8508
dc.language.isoeng
dc.publisherAmer Chemical Soc
dc.relation.ispartofLangmuir
dc.sourceWeb of Science
dc.titleFabrication of phytic acid sensor based on mixed phytase-lipid Langmuir-Blodgett filmsen
dc.typeArtigo
dcterms.licensehttp://pubs.acs.org/paragonplus/copyright/jpa_form_a.pdf
dcterms.rightsHolderAmer Chemical Soc
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.description.affiliationUniv São Paulo, Grp Polimeros, Inst Fis Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Fac Ciências & Tecnol, Presidente Prudente, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Filosofia Ciências & Letras Ribeirao Pret, Dept Quim, BR-14049 Ribeirao Preto, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim Sao Carlos, Sao Carlos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências & Tecnol, Presidente Prudente, SP, Brazil
dc.identifier.doi10.1021/la061799g
dc.identifier.wosWOS:000240573200038
dc.rights.accessRightsAcesso restrito
unesp.author.orcid0000-0001-6913-8540[7]
unesp.author.orcid0000-0003-4307-3077[3]
unesp.author.orcid0000-0003-0213-3686[2]
unesp.author.orcid0000-0002-9459-8167[4]
unesp.author.orcid0000-0002-5399-5860[8]
dc.relation.ispartofjcr3.789
dc.relation.ispartofsjr1,479
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