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dc.contributor.authorNakaie, Clovis R.
dc.contributor.authorMalavolta, Luciana
dc.contributor.authorSchreier, Shirley
dc.contributor.authorTrovatti, Eliane
dc.contributor.authorMarchetto, Reinaldo [UNESP]
dc.date.accessioned2014-05-20T15:30:15Z
dc.date.available2014-05-20T15:30:15Z
dc.date.issued2006-06-14
dc.identifierhttp://dx.doi.org/10.1016/j.polymer.2006.05.007
dc.identifier.citationPolymer. Oxford: Elsevier B.V., v. 47, n. 13, p. 4531-4536, 2006.
dc.identifier.issn0032-3861
dc.identifier.urihttp://hdl.handle.net/11449/39675
dc.description.abstractThis work demonstrates, for the first time. a time-resolved electron paramagnetic resonance (EPR) monitoring of a chemical reaction occurring in a polymeric structure. The progress of the coupling of a N-alpha-tert-butyloxycarbonyl-2.2.6.6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid (Boc-TOAC) spin probe to a model peptide-resin was followed through EPR spectra. Progressive line broadening of EPR peaks was observed, indicative of an increased population of immobilized spin probe molecules attached to the solid support. The time for spectral stabilization of this process coincided with that determined in a previous Coupling study. thereby validating this in situ quantitative monitoring of the reaction. In addition, the influence of polymer swelling degree and solvent viscosity, as well as of the steric hindrance within beads. on the rate of coupling reaction was also addressed. A deeper evaluation of the latter effect was possible by determining unusual polymer parameters such as the average site-site distance and site-concentration within resin beads in each solvent system. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.format.extent4531-4536
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofPolymer
dc.sourceWeb of Science
dc.subjectpeptidept
dc.subjectpolymerpt
dc.subjectelectron paramagnetic resonancept
dc.titleDirect electron paramagnetic resonance monitoring of the peptide synthesis coupling reaction in polymeric supporten
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.description.affiliationUniv Fed São Paulo, Dept Biophys, BR-04044020 São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Chem, Dept Biochem, BR-05513970 São Paulo, Brazil
dc.description.affiliationUNESP, Dept Biochem & Technol Chem, Inst Chem, BR-14800900 São Paulo, Brazil
dc.description.affiliationUnespUNESP, Dept Biochem & Technol Chem, Inst Chem, BR-14800900 São Paulo, Brazil
dc.identifier.doi10.1016/j.polymer.2006.05.007
dc.identifier.wosWOS:000238921300010
dc.rights.accessRightsAcesso restrito
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
dc.identifier.lattes5711182251641103
unesp.author.lattes5711182251641103
dc.relation.ispartofjcr3.483
dc.relation.ispartofsjr1,097
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