Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii
Nenhuma Miniatura disponível
Data
2009-05-01
Autores
Devitt, Thomas J.
Pereira, Ricardo
Jakkula, Lakshmi
Alexandrino, Joao [UNESP]
Bardeleben, Carolyne
Moritz, Craig
Título da Revista
ISSN da Revista
Título de Volume
Editor
Wiley-Blackwell Publishing, Inc
Resumo
We developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)(n) repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.
Descrição
Palavras-chave
Ensatina, microsatellite, Plethodontidae, plethodontid, ring species, salamander
Como citar
Molecular Ecology Resources. Malden: Wiley-blackwell Publishing, Inc, v. 9, n. 3, p. 966-969, 2009.