A Preliminary and Qualitative Metallomics Study of Mercury in the Muscle of Fish from Amazonas, Brazil
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Data
2012-12-01
Autores
Moraes, Paula M. [UNESP]
Santos, Felipe A. [UNESP]
Padilha, Cilene C. F. [UNESP]
Vieira, Jose C. S. [UNESP]
Zara, Luiz F.
Padilha, Pedro de M. [UNESP]
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Editor
Humana Press Inc
Resumo
This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish species from Amazonas, Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis and subsequent evaluation of mercury by synchrotron radiation X-ray fluorescence. The fluorescence spectra revealed mercury in two protein spots. The mercury-containing protein spots showed molecular weights of 20.8 +/- 0.7 and 19.8 +/- 0.5 kDa and isoelectric points of 5.6 +/- 0.2 and 7.5 +/- 0.3, respectively.
Descrição
Palavras-chave
Metallomics study, Mercury in fish, 2D PAGE, SR XRF
Como citar
Biological Trace Element Research. Totowa: Humana Press Inc, v. 150, n. 1-3, p. 195-199, 2012.